Biodegradation of thiocyanate using co-culture of Klebsiella pneumoniae and Ralstonia sp
| Autor: | Ashvini U. Chaudhari, Kisan M. Kodam |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Oxygenase Thiocyanate hydrolase Nitrogen Klebsiella pneumoniae Industrial Waste Ralstonia Applied Microbiology and Biotechnology chemistry.chemical_compound RNA Ribosomal 16S Soil Microbiology chemistry.chemical_classification biology Thiocyanate Temperature General Medicine Hydrogen-Ion Concentration Biodegradation biology.organism_classification Carbon Coculture Techniques Enzyme assay Culture Media Biodegradation Environmental Enzyme chemistry Biochemistry biology.protein Thiocyanates Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 85:1167-1174 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Thiocyanate-degrading microbial co-culture was isolated from thiocyanate-contaminated site and tested for thiocyanate degradation potential and thiocyanate-toxicity tolerance and identified as Klebsiella pneumoniae and Ralstonia sp. by 16S rDNA sequencing. The co-culture was able to degrade thiocyanate with degradation rate of 500 mg L(-1)d(-1) at 2,500 mg L(-1) thiocyanate concentration at pH 6.0 and 37 degrees C following thiocyanate hydrolase pathway. The Haldane kinetic model elucidates the growth and thiocyanate biodegradation kinetics of the co-culture with Ki value of 1,876 mg L(-1). The thiocyanate biodegradation kinetics was not affected by the additional supply of glucose. The very high activities of thiocyanate hydrolase, cyanide oxygenase, and cytochrome P-450 content during growth on thiocyanate were observed, showing the induction mechanism. |
| Databáze: | OpenAIRE |
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