| Popis: |
By application of cytochalasin D (10 micrograms/ml) the distribution and turnover of endoplasmic reticulum (ER) in root statocytes of cress (Lepidium sativum L.) was studied. After 7 min of incubation, the distal ER complex, in 20-h-old control statocytes consisting of stacked cisternae, was disintegrated and redistributed. The amyloplasts sedimented into the most distal part of the cell. When the incubation time was increased up to 4 h, ER was formed near the nucleus and accumulated at the proximal cell pole. Thus microfilaments are suggested to be involved in (i) stabilization of the distal ER complex and (ii) the ER translocation from the forming site into the distal cell pole. By morphometric measurements the volume of story II (story III) statocytes was calculated to be 2400 microns3 (3000 microns3), containing an ER area of 1824 microns2 (2400 microns2). From the net ER formation rate of 5.2 microns2/min (story II) and 4.6 microns2/min (story III) and the net decrease rate of 23 microns2/min (story II) and 39.2 microns2/min (story III), a total ER formation rate of about 27 microns2/min (story II) and 43 microns2/min (story III) was estimated. |
