Augmented secretion of IL-1α from mouse oral squamous cell carcinoma (OSCC) vcells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells
| Autor: | Shinichiro Sumitomo, Yasunori Muramatsu, Masako Mizuno-Kamiya, Kyohei Ueno, Megumi Ando, Nobuo Kondoh, Akihiro Matsunami, Harumi Kawaki, Hiroe Morimoto-Ito, Eiji Takayama |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Stromal cell Medicine (miscellaneous) Spleen General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice 0302 clinical medicine Immune system medicine Animals Hypoxia General Dentistry biology Chemistry Squamous Cell Carcinoma of Head and Neck Mesenchymal stem cell Interleukin Mesenchymal Stem Cells 030206 dentistry Squamous carcinoma 030104 developmental biology medicine.anatomical_structure Head and Neck Neoplasms Cancer research biology.protein Carcinoma Squamous Cell Immunohistochemistry Mouth Neoplasms Antibody |
| Zdroj: | Journal of oral biosciences. 63(3) |
| ISSN: | 1880-3865 |
| Popis: | Objectives We have previously reported that mouse oral squamous carcinoma (OSCC), Sq-1979-1, produces interleukin (IL)-1α, which specifically enhances the immunosuppressive activity of co-cultured mesenchymal stromal 10T1/2 cells. This study assessed the conditions promoting the production of IL-1α in Sq-1979-1 cells, which could further enhance the immunosuppressive function of 10T1/2 cells, and evaluated its expression in OSCC tissues. Methods The expression of IL-1α was examined by RT-PCR, western blotting, and enzyme-linked immune sorbent assay (ELISA). The interferon (IFN)- γ-producing capability of anti-CD3 antibody-stimulated mouse spleen cells co-cultured with 10T1/2 cells and conditioned medium (CM) from Sq-1979-1 cells was examined by ELISA. The function of IL-1α was examined using an anti-IL1α antibody. Immunohistochemical analysis of the OSCC tissues was performed. Results The production of IL-1α from Sq-1979-1 cells was synergistically enhanced in lower serum (0.5% or 1.0% FBS) at the transcriptional level, and under hypoxia (1.0% oxygen) at the release level compared to that in the control medium supplemented with 10% FBS under normoxia. The IFN-γ-producing capability of stimulated spleen cells co-cultured with 10T1/2 cells was significantly reduced in the CMs prepared with the lower serum or under hypoxia. These functions of CMs were completely abolished by the anti-IL-1α antibody. The expression of IL-1α in OSCC tissues was prominent in the midst of a carcinomatous cellular lesion or a nearby necrotic lesion, where a supply deficiency could occur. Conclusion s: IL-1α production by Sq-1979-1 cells was synergistically augmented under low serum and hypoxic conditions, which could promote the immunosuppressive activity of mesenchymal cells. |
| Databáze: | OpenAIRE |
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