Synthesis and circularization of N- and B-tropic retroviral DNA Fv-1 permissive and restrictive mouse cells
| Autor: | Wen K. Yang, Chin-Yih Ou, Robert H. Bassin, Raymond W. Tennant, Den-Mei Yang, Arthur B. Brown, James O. Kiggans |
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| Rok vydání: | 1980 |
| Předmět: |
viruses
Virus Replication Virus chemistry.chemical_compound Mice Retrovirus Genes Regulator Animals Gene Cells Cultured Multidisciplinary biology DNA replication biology.organism_classification Virology Molecular biology Leukemia Virus Murine Kinetics chemistry Viral replication Cell culture Agarose gel electrophoresis DNA Viral DNA Circular DNA Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 77(5) |
| ISSN: | 0027-8424 |
| Popis: | Production of various forms of nonintegrated viral DNA was measured in cultured mouse cells carrying different Fv-1 alleles early after infection with N-tropic or B-tropic retroviruses. Quantitative analyses were performed by agarose gel electrophoresis, transfer to diazobenzyloxymethyl-paper, and molecular hybridization. In permissive infection of Fv-1n cells (NIH Swiss and DBA mouse strains) with N-tropic virus and of Fv-1b cells (BALB/c and C57BL/6 strains) with B-tropic virus, form III (double-stranded linear) DNA first appeared at 3-4 hr and reached a maximum at 8-10 hr; two form I (closed circle) DNAs appeared at 7-8 hr and reached a maximum at or beyond 12 hr. In the two Fv-1b cells infected with N-tropic virus and in DBA (Fv-1n) cells infected with B-tropic virus, formation of the two form I DNAs was quantitatively restricted but formation of form III DNA was unaltered. In Fv-1n NIH Swiss mouse embryo cells infected with B-tropic virus, the level of form III DNA was markedly depressed and hence the two form I DNAs were not detectable. In C57BL/6 cells as well as in DBA/2 cells 12 hr after infection, the quantity of form III DNA varied directly with the amount of restricted virus, whereas the quantity of form I DNA varied according to the square of the amount of restricted virus. The significance of these results for understanding the molecular basis of retrovirus replication and its restriction by the Fv-1 gene is discussed. |
| Databáze: | OpenAIRE |
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