Dynamic modulation of Cyp21a1 (21-hydroxylase) expression sites in the mouse developing lung

Autor: Catherine Gilbert, Pierre R. Provost, Yves Tremblay
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Pathology
Endocrinology
Diabetes and Metabolism
Mesenchyme
Clinical Biochemistry
In situ hybridization
Biology
Biochemistry
Epithelium
Mice
03 medical and health sciences
Receptors
Glucocorticoid
0302 clinical medicine
Endocrinology
Glucocorticoid receptor
Internal medicine
medicine
Animals
Humans
Desoxycorticosterone
Glucocorticoids
Lung
Molecular Biology
In Situ Hybridization
Mice
Inbred BALB C
Gene Expression Profiling
Tunica Adventitia
21-Hydroxylase
Gene Expression Regulation
Developmental
Epithelial Cells
Cell Biology
Pulmonary Alveoli
030104 developmental biology
medicine.anatomical_structure
Dynamic modulation
Alveolar Epithelial Cells
030220 oncology & carcinogenesis
biology.protein
Molecular Medicine
Female
Steroid 21-Hydroxylase
Zdroj: The Journal of Steroid Biochemistry and Molecular Biology. 168:102-109
ISSN: 0960-0760
DOI: 10.1016/j.jsbmb.2017.02.009
Popis: 21-hydroxylase is expressed in the developing lung where it is proposed as a local source of glucocorticoids playing important roles in lung development. We have studied the precise sites of Cyp21a1 expression in the developing mouse lung from the pseudoglandular stage (gestation day (GD) 15.5) to the alveolar stage (postnatal day (PND) 15) by in situ hybridization. Cyp21a1-mRNA was found mainly in epithelial cells from GD 15.5 to PND 5, but the precise site of expression shifted from the distal epithelium during the pseudoglandular and the canalicular stages including the distal epithelium without lumina, to the proximal epithelium and the wall of developing saccules during the perinatal period (GD 19.5 and PND 0), and to the wall of developing saccules and septa, most probably in type I pneumonocytes (PTI), on PND 5. Cyp21a1 expression changed from PTI cells to capillary endothelial cells of the same distal structures during alveolarization. The mesenchyme was generally negative. Endothelial cells forming large vessels were negative. However the tunica adventitia surrounding arteries was Cyp21a1-positive, while several veins were surrounded by a Cyp21a1-positive layer. In conclusion, Cyp21a1 remains expressed in the most distal structure of the developing lung even though these structures are changing, but its expression is not restricted to these areas. Taken together, our data show the highly dynamic modulation of Cyp21a1 expression sites, consistent with the evolving structures of the developing lung.
Databáze: OpenAIRE
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