Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy
| Autor: | Arnab Chakraborty, Fabien Deligey, Tuo Wang, Jenny Quach, Frederic Mentink-Vigier, Ping Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Nanotechnology
Ligands 010402 general chemistry 01 natural sciences Biochemistry Article 03 medical and health sciences Biopolymers Cell Wall Polysaccharides Humans Spectroscopy Nuclear Magnetic Resonance Biomolecular 030304 developmental biology Cell Nucleus chemistry.chemical_classification 0303 health sciences Bacteria Biomolecule Cell Membrane Fungi Temperature technology industry and agriculture Membrane Proteins Biomaterial Plants 0104 chemical sciences chemistry Solid-state nuclear magnetic resonance Biomolecular complex Protein Binding |
| Zdroj: | Biochem Soc Trans |
| ISSN: | 1470-8752 0300-5127 |
| Popis: | Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. The recent advances in the sensitivity-enhancing technique magic-angle spinning dynamic nuclear polarization (MAS-DNP) have substantially expanded the territory of ssNMR investigations and enabled the detection of polymer interfaces in a cellular environment. This article highlights the emerging MAS-DNP approaches and their applications to the analysis of biomolecular composites and intact cells to determine the folding pathway and ligand binding of proteins, the structural polymorphism of low-populated biopolymers, as well as the physical interactions between carbohydrates, proteins, and lignin. These structural features provide an atomic-level understanding of many cellular processes, promoting the development of better biomaterials and inhibitors. It is anticipated that the capabilities of MAS-DNP in biomolecular and biomaterial research will be further enlarged by the rapid development of instrumentation and methodology. |
| Databáze: | OpenAIRE |
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