Generation of Lungs by Blastocyst Complementation in Apneumic Fgf10-Deficient Mice
| Autor: | Toshikuni Sasaoka, Manabu Abe, Kanako Oda, Akihiro Yasue, Yoichi Ajioka, Xulu Ye, Yasuo Saijo, Kenji Sakimura, Akihiko Kitahara, Qiliang Zhou, Qingsong Ran, Masanori Tsuchida |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Connective tissue Biology Chimerism General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice 0302 clinical medicine Parenchyma medicine Lung transplantation Animals Blastocyst Induced pluripotent stem cell Lung reproductive and urinary physiology urogenital system respiratory system Embryonic stem cell Cell biology Complementation Disease Models Animal 030104 developmental biology medicine.anatomical_structure embryonic structures Fibroblast Growth Factor 10 030217 neurology & neurosurgery |
| Zdroj: | Cell reports. 31(6) |
| ISSN: | 2211-1247 |
| Popis: | The shortage of donor lungs hinders lung transplantation, the only definitive option for patients with end-stage lung disease. Blastocyst complementation enables the generation of transplantable organs from pluripotent stem cells (PSCs) in animal models. Pancreases and kidneys have been generated from PSCs by blastocyst complementation in rodent models. Here, we report the generation of lungs using mouse embryonic stem cells (ESCs) in apneumic Fgf10 Ex1mut/Ex3mutmice by blastocyst complementation. Complementation with ESCs enables Fgf10-deficient mice to survive to adulthood without abnormalities. Both the generated lung alveolar parenchyma and the interstitial portions, including vascular endothelial cells, vascular and parabronchial smooth muscle cells, and connective tissue, largely originate from the injected ESCs. These data suggest that Fgf10 Ex1mut/Ex3mutblastocysts provide an organ niche for lung generation and that blastocyst complementation could be a viable approach for generating whole lungs. |
| Databáze: | OpenAIRE |
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