Outbreak of equine pythiosis in a southeastern region of Brazil: Environmental isolation and phylogeny
| Autor: | Sandra de Moraes Gimenes Bosco, José Eduardo Cury, Jéssica Luana Chechi, Emanuel Vitor Pereira Apolonio, Eduardo Bagagli, Hans Garcia Garces, Alana Lucena Oliveira, Gabriel Gasparini Camargo, Marcos Jun Watanabe, Ana Carolina do Prado, Giselle Souza da Paz |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Autonomous Veterinarian |
| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
Pythium insidiosum Pythium Disease Outbreaks Pythiosis Dogs Phylogenetics Animals ecological niche Horses Clade Phylogeny ozone therapy Oomycete General Veterinary General Immunology and Microbiology Phylogenetic tree biology Outbreak General Medicine biology.organism_classification oomycetes horse Horse Diseases Nested polymerase chain reaction Brazil |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1865-1682 |
| Popis: | Made available in DSpace on 2021-06-25T11:18:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Pythiosis is a disease caused by the oomycete Pythium insidiosum, mainly reported in equines, dogs and humans and directly transmitted through contaminant zoospores in aquatic environments. We report the first outbreak of equine pythiosis in five equines. Wound samples were submitted for diagnostic testing including mycological culture and nested PCR. Treatment approaches consisted of conventional and alternative therapies. Microbiological analyses were performed using water samples from the riverbanks close to where the animals had grazed. All animals were positive for P. insidiosum cultures, and two animals responded successfully to alternative therapy (ozone therapy). After culture and molecular analysis of environmental samples, the presence of P. insidiosum in one section of the Tietê River was confirmed through a 99% sequence identity. Phylogenetic analyses using the cytochrome oxidase II gene showed that the animal isolates clustered in clade I and the environmental isolates clustered in clade III. Although the environmental and wound isolates belonged to different genetic clades, we concluded that the Tietê River is an important source of infection by P. insidiosum and that research concerning environmental isolation of P. insidiosum from rivers and lakes should be strongly facilitated in Brazil. School of Veterinary Medicine and Animal Science UNESP Univ Estadual Paulista Institute of Biosciences of Botucatu UNESP Univ Estadual Paulista Autonomous Veterinarian School of Veterinary Medicine and Animal Science UNESP Univ Estadual Paulista Institute of Biosciences of Botucatu UNESP Univ Estadual Paulista |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|