NMR solution structure of a DNA dodecamer containing a transplatin interstrand GN7-CN3 cross-link
| Autor: | Gérard Lancelot, Françoise Paquet, Marc Leng, Marc Boudvillain |
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| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), LEGOUPIL, Laëtitia, Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1999 |
| Předmět: |
Models
Molecular Base pair [SDV]Life Sciences [q-bio] Cytosine Nucleotides Biology 010402 general chemistry 01 natural sciences ACTIVATION DNA Adducts CISPLATIN 03 medical and health sciences Cytosine nucleotide BINDING [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Genetics Moiety [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology A-DNA Base Pairing Nuclear Magnetic Resonance Biomolecular Platinum 030304 developmental biology CIS 0303 health sciences Base Sequence Hydrogen bond Hydrogen Bonding DNA Guanine Nucleotides APOPTOSIS 0104 chemical sciences Solutions [SDV] Life Sciences [q-bio] Kinetics Crystallography Cross-Linking Reagents Dodecameric protein Oligodeoxyribonucleotides Biochemistry Duplex (building) Nucleic Acid Conformation Protons PLATINUM ANTITUMOR COMPLEXES Two-dimensional nuclear magnetic resonance spectroscopy Software FORM Research Article |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 1999, 27 (21), pp.4261-4268. ⟨10.1093/nar/27.21.4261⟩ Nucleic Acids Research, 1999, 27 (21), pp.4261-4268. ⟨10.1093/nar/27.21.4261⟩ |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | International audience; The DNA duplex d(CTCTCG*AGTCTC)d(GAGACTC*GAGAG) containing a single trans-diammine-dichloroplatinum(ll) interstrand cross-link (where G* and C* represent the platinated bases) has been studied by two-dimensional NMR, All the exchangeable and non-exchangeable proton resonance lines were assigned (except H5'/H5 ") and the NOE intensities were transformed into distances via the RELAZ program. By combining the NOESY and COSY data (330 constraints) and NMR-constrained molecular mechanics using JUMNA, a solution structure of the cross-linked duplex has been determined. The duplex is distorted over two base pairs on each side of the interstrand cross-link and exhibits a slight bending of its axis (similar to 20 degrees) towards the minor groove. The platinated guanine G* adopts a syn conformation, The rotation results in a Hoogsteen-type pairing between the complementary G(6)* and C-19* residues which is mediated by the platinum moiety and is stabilized by a hydrogen bond between O6(G(6)*) and N4H(C-19*). The rise between the cross-linked residues and the adjacent residues is increased owing to the interaction between these adjacent residues and the ammine groups of the platinum moiety, These results are discussed in relation to the slow rate of closure of the monofunctional adducts into interstrand cross-links. |
| Databáze: | OpenAIRE |
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