Photosensitizers attenuate LPS-induced inflammation: implications in dentistry and general health
| Autor: | Ana Carolina Fernandes Couto, Paulo Nelson-Filho, Gilberto U.L. Braga, Raquel Assed Bezerra da Silva, Francisco Wanderley Garcia Paula-Silva, Renan Villanova Homem de Carvalho, Guilherme T.P. Brancini, Maria Cristina Borsatto, Arthur Belém Novaes Júnior, Carlos Arterio Sorgi, Fernando Grine Martins |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharide
Inflammation Context (language use) New methylene blue INFLAMAÇÃO 030206 dentistry Dermatology Pharmacology Proinflammatory cytokine stomatognathic diseases 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry In vivo Limulus amebocyte lysate medicine Curcumin lipids (amino acids peptides and proteins) Surgery medicine.symptom |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Antimicrobial photodynamic therapy (aPDT) is a complementary therapeutic modality for periodontal and endodontic diseases, in which Gram-negative bacteria are directly involved. Currently, there are few evidences regarding the effects of aPDT on bacterial components such as lipopolysaccharide (LPS) and it would represent a major step forward in the clinical use of this therapy. In this context, this study aimed to evaluate the efficacy of different photosensitizers (PSs) used in aPDT in LPS inhibition. Four PSs were used in this study: methylene blue (MB), toluidine blue (TBO), new methylene blue (NMB), and curcumin (CUR). Different approaches to evaluate LPS interaction with PSs were used, such as spectrophotometry, Limulus amebocyte lysate (LAL) test, functional assays using mouse macrophages, and an in vivo model of LPS injection. Spectrophotometry showed that LPS decreased the absorbance of all PSs used, indicating interactions between the two species. LAL assay revealed significant differences in LPS concentrations upon pre-incubation with the different PSs. Interestingly, the inflammatory potential of LPS decreased after previous treatment with the four PSs, resulting in decreased secretion of inflammatory cytokines by macrophages. In vivo, pre-incubating curcumin with LPS prevented animals from undergoing septic shock within the established time. Using relevant models to study the inflammatory activity of LPS, we found that all PSs used in this work decreased LPS-induced inflammation, with a more striking effect observed for NMB and curcumin. These data advance the understanding of the mechanisms of LPS inhibition by PSs. |
| Databáze: | OpenAIRE |
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