Abscisic acid plasmalemma perception triggers a calcium influx essential forRAB18gene expression inArabidopsis thalianasuspension cells
| Autor: | Emile Miginiac, Françoise Bardat, Emmanuelle Jeannette, Bruno Sotta, Thanos Ghelis, Olivier Dellis |
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| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Arabidopsis Biophysics chemistry.chemical_element Biology Calcium Biochemistry chemistry.chemical_compound Gene Expression Regulation Plant Structural Biology Gene expression Genetics Extracellular Arabidopsis thaliana Molecular Biology Abscisic acid Plant Proteins Calcium metabolism Dose-Response Relationship Drug Arabidopsis Proteins Cell Membrane fungi food and beverages Biological Transport Cell Biology Blotting Northern biology.organism_classification Arabidopsis thaliana suspension cells Molecular biology Cell biology EGTA chemistry RNA Plant rab GTP-Binding Proteins Calcium channel inhibitor Signal transduction Extracellular Space Abscisic acid transduction Abscisic Acid |
| Zdroj: | FEBS Letters. 483:67-70 |
| ISSN: | 0014-5793 |
| Popis: | Pretreatment of Arabidopsis thaliana suspension cells with impermeant calcium chelator EGTA inhibited the ABA-induced RAB18 gene expression. However, extracellular calcium alone, up to 10 mM, did not trigger RAB18 expression. Spectrofluorimetric extracellular Ca 2+ measurement with Fluo-3 showed a fast, within 1 min, Ca 2+ influx associated with outer plasmalemma ABA perception. In the presence of the Ca 2+ blockers Cd 2+ and Ni 2+ , RAB18 expression was suppressed. Pimozide and fluspirilene inhibited Ca 2+ influx and ABA-induced RAB18 expression. Thus we demonstrated the involvement of specific Ca 2+ influx in the ABA signaling sequence leading to RAB18 expression. |
| Databáze: | OpenAIRE |
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