Rosettes integrity protects Plasmodium vivax of being phagocytized
| Autor: | Marcus V. G. Lacerda, Ana Beatriz Iung Enembreck da Silva, Rodrigo Pm de Almeida, Luiz Fernando Cardoso Garcia, Fabio T. M. Costa, Letusa Albrecht, Catarina Bourgard, Ana Carolina A. V. Kayano, Juliana A. Leite, André M. Siqueira, Bruce Russell, Najara C. Bittencourt, Laurent Rénia, Irene S. Soares, Hellen Geremias dos Santos, Stefanie C. P. Lopes, Vanessa de Araújo Barbosa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Rosette Formation Science Phagocytosis 030231 tropical medicine Plasmodium vivax Parasitemia Phagocytosis Pathway Article Pathogenesis 03 medical and health sciences 0302 clinical medicine Immune system parasitic diseases Parasite immune evasion Malaria Vivax medicine Humans MALÁRIA Multidisciplinary biology Interleukin-6 biology.organism_classification medicine.disease Phenotype Malaria Interleukin-10 030104 developmental biology Immunoglobulin M Immunology Medicine Ex vivo |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2045-2322 |
| Popis: | Plasmodium vivax is the most prevalent cause of malaria outside of Africa. P. vivax biology and pathogenesis are still poorly understood. The role of one highly occurring phenotype in particular where infected reticulocytes cytoadhere to noninfected normocytes, forming rosettes, remains unknown. Here, using a range of ex vivo approaches, we showed that P. vivax rosetting rates were enhanced by plasma of infected patients and that total immunoglobulin M levels correlated with rosetting frequency. Moreover, rosetting rates were also correlated with parasitemia, IL-6 and IL-10 levels in infected patients. Transcriptomic analysis of peripheral leukocytes from P. vivax-infected patients with low or moderated rosetting rates identified differentially expressed genes related to human host phagocytosis pathway. In addition, phagocytosis assay showed that rosetting parasites were less phagocyted. Collectively, these results showed that rosette formation plays a role in host immune response by hampering leukocyte phagocytosis. Thus, these findings suggest that rosetting could be an effective P. vivax immune evasion strategy. |
| Databáze: | OpenAIRE |
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