Improved liquid-liquid extraction by modified magnetic nanoparticles for the detection of eight drugs in human blood by HPLC-MS
| Autor: | Ke Ma, Feiyu Yang, Yu Cao, Chunfang Ni |
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| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Chemistry Scanning electron microscope General Chemical Engineering 010401 analytical chemistry Energy-dispersive X-ray spectroscopy Infrared spectroscopy Ion suppression in liquid chromatography–mass spectrometry General Chemistry Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences 0302 clinical medicine Liquid–liquid extraction Magnetic nanoparticles 030216 legal & forensic medicine |
| Zdroj: | RSC advances. 11(32) |
| ISSN: | 2046-2069 |
| Popis: | Magnetic nanoparticles modified with porous titanium dioxide were used as clean-up nanospheres for the detection of eight drug poisons in human blood by high-performance liquid chromatography-mass spectrometry. The magnetic clean-up nanospheres (Fe3O4@mTiO2) with a mesoporous structure were successfully synthesized and characterized by scanning electron microscopy/energy dispersive spectroscopy, transmission electron microscopy, X-ray diffractometry, vibrating sample magnetometry, infrared spectroscopy, and Brunauer–Emmett–Teller techniques. Lipid co-extractives, such as phosphatidic acid and fatty acids, which are major interferences in HPLC-MS analysis causing ion suppression in the MS spectra of blood, could be efficiently removed by Fe3O4@mTiO2 based on the Lewis acid–Lewis base interactions. Following the optimization of the quantities of Fe3O4@mTiO2, the method was applied to the determination of eight drugs in spiked blood. The analytical ranges typically extended from 2 to 500 ng mL−1, and the recoveries ranged from 79.5–99.9% at different concentrations of blood. The limits of quantitation for drug poisons were 0.14–1.03 ng mL−1, which makes the method a viable tool for drug poison monitoring in blood. |
| Databáze: | OpenAIRE |
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