Enzymatically induced mineralization of platelet-rich fibrin
| Autor: | Volker Gassling, Heidi Declercq, Elżbieta Pamuła, Timothy E.L. Douglas, Nicolai Purcz, Eric L.W. de Mulder, John A. Jansen, Håvard J. Haugen, Safak Chasan, Sander C.G. Leeuwenburgh |
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| Rok vydání: | 2012 |
| Předmět: |
Blood Platelets
Materials science Biomedical Engineering chemistry.chemical_element Calcium Mineralization (biology) Fibrin Biomaterials Calcification Physiologic Cell Line Tumor Spectroscopy Fourier Transform Infrared Animals Humans Von Kossa stain Cell Death Staining and Labeling biology Metals and Alloys Spectrometry X-Ray Emission Biomaterial Alkaline Phosphatase digestive system diseases Platelet-rich fibrin Staining Freeze Drying Microscopy Fluorescence Biochemistry chemistry Thermogravimetry Microscopy Electron Scanning Ceramics and Composites biology.protein Alkaline phosphatase Cattle Nuclear chemistry |
| Zdroj: | Journal of Biomedical Materials Research Part A. :1335-1346 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.34073 |
| Popis: | Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, EDS, FTIR, and von Kossa staining. The mass percentage attributable to CaP was quantified by lyophilization and measurement of the remaining mass fraction as well as by TGA. Cytocompatibility tests (LDH, MTT, and WST) with SAOS-2 cells showed that mineralized PRF did not release substances detrimental to cell vitality. Live/dead staining and SEM showed that mineralized PRF was colonized by cells. The results show that hydrogel biomaterials such as PRF can be mineralized through functionalization with ALP. |
| Databáze: | OpenAIRE |
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