Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
| Autor: | Rikako Shirai, Nobumitsu Sasaki, Kazuo Taku, Richard S. Nelson, Hiroshi Nyunoya, Tsumugi Miyazaki, Yasuhiko Matsushita, Chihiro Ikeda |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant molecular biology Science viruses Transgene Gene Expression Nicotiana benthamiana Biology Genes Plant 01 natural sciences Article Virus 03 medical and health sciences Gene Expression Regulation Plant Tobacco Gene expression Tobacco mosaic virus Transgenes Promoter Regions Genetic Biotic Gene Disease Resistance Plant Diseases Plant Proteins Multidisciplinary Cell Death Intron food and beverages Plants Genetically Modified biology.organism_classification Molecular biology Introns Elicitor Tobacco Mosaic Virus 030104 developmental biology Host-Pathogen Interactions Medicine 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-94713-4 |
| Popis: | The tobacco virus resistance gene N contains four introns. Transient expression of transcripts from an N transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the native promoter, the elicitor, or the individual introns for enhanced expression of N has not been fully studied. Here, we determined that 35S promoter-driven N transcript expression could be enhanced in the presence of the four introns regardless of the co-expression of the virus elicitor in tobacco. Function analyses using a series of N transgenes with different combination of introns revealed that the presence of intron 1 more so than intron 2 allowed higher accumulation of premature and mature N transcripts; however, both introns were important for not only enhanced gene expression but also for induction of cell death in tobacco and induced local resistance to spread of virus in Nicotiana benthamiana. Our findings indicate that introns 1 and 2 cooperatively contribute to N expression and virus resistance. |
| Databáze: | OpenAIRE |
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