EMS Mutagenesis in the Pea AphidAcyrthosiphon pisum
| Autor: | Gaël Le Trionnaire, Jean-Pierre Gauthier, Denis Tagu, Sylvie Tanguy, Jean-René Huynh |
|---|---|
| Přispěvatelé: | Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), ProdInra, Archive Ouverte, AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2014 |
| Předmět: |
Male
éthyl méthanesulfonate 0106 biological sciences Mutation rate mutation génétique Genome Insect Mutant medicine.disease_cause 01 natural sciences chemistry.chemical_compound chromosome x Wings Animal [SDV.BV] Life Sciences [q-bio]/Vegetal Biology mutagenèse Genetics (clinical) Genetics 0303 health sciences Mutation Vegetal Biology biology EMS food and beverages X-linked mutation larve espèce parthénogénétique puceron Ethyl Methanesulfonate Larva Female génotype Drosophila melanogaster pea aphid mutagenesis X Chromosome Genotype Ethyl methanesulfonate plasticité phénotypique Mutagenesis (molecular biology technique) Investigations acyrthosiphon pisum 03 medical and health sciences Genetic model medicine Animals [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Molecular Biology 030304 developmental biology biology.organism_classification Acyrthosiphon pisum chemistry Aphids Biologie végétale 010606 plant biology & botany |
| Zdroj: | G3 G3, Genetics Society of America, 2014, 4, pp.657-667. ⟨10.1534/g3.113.009639⟩ G3, 2014, 4, pp.657-667. ⟨10.1534/g3.113.009639⟩ G3-Genes Genomes Genetics (4), 657-667. (2014) G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | International audience; In aphids, clonal individuals can show distinct morphological traits in response to environmental cues. Such phenotypic plasticity cannot be studied with classical genetic model organisms such as [i]Caenorhabditis elegans[/i] or [i]Drosophila melanogaster[/i]. The genetic basis of this biological process remain unknown, as mutations affecting this process are not available in aphids. Here, we describe a protocol to treat third stage larvae with an alkylating mutagen, ethyl methanesulfonate (EMS), to generate random mutations within the [i]Acyrthosiphon pisum[/i] genome. We found that even low concentrations of EMS were toxic for two genotypes of [i]A. pisum[/i]. Mutagenesis efficiency was nevertheless assessed by estimating the occurrence of mutational events on the X chromosome. Indeed, any lethal mutation on the X-chromosome would kill males which are haploid on the X so that we used the proportion of males as an estimation of mutagenesis efficacy. We could assess a putative mutation rate of 0.4 per X-chromosome at 10 mM of EMS. We then applied this protocol to perform a small scale mutagenesis on parthenogenetic individuals, which were screened for defects in their ability to produce sexual individuals in response to photoperiod shortening. We found one mutant line showing a reproducible altered photoperiodic response with a reduced production of males and the appearance of aberrant winged males (wing atrophy, alteration of legs morphology). This mutation appeared to be stable since it could be transmitted over several generations of parthenogenetic individuals. To our knowledge, this study represents the first example of an EMS-generated aphid mutant. |
| Databáze: | OpenAIRE |
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