An immuno-dot blot assay for screening histidine kinase inhibitors
| Autor: | Ryutaro Utsumi, Yoko Eguchi, Masayuki Igarashi, Eiji Kinoshita, Tohru Koike, Emiko Kinoshita-Kikuta, Toshihide Okajima, Shino Maruta |
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| Rok vydání: | 2020 |
| Předmět: |
Histidine Kinase
Biophysics Dot blot Virulence 01 natural sciences Biochemistry 03 medical and health sciences Structure-Activity Relationship Molecular Biology Protein Kinase Inhibitors 030304 developmental biology 0303 health sciences biology Dose-Response Relationship Drug Chemistry 010401 analytical chemistry Histidine kinase Quinones Cell Biology biology.organism_classification 0104 chemical sciences High-Throughput Screening Assays Blot Response regulator biology.protein Antibody Signal transduction Bacteria Signal Transduction |
| Zdroj: | Analytical biochemistry. 600 |
| ISSN: | 1096-0309 |
| Popis: | Two-component signal transduction systems (TCSs), consisting of a histidine kinase (HK) and its cognate response regulator, are ubiquitous among bacteria and are associated with the virulence of pathogens. TCSs are potential targets for alternative antibiotics and antivirulence agents. It is, thus, very important to determine HK activity in bacterial TCSs. Here, we describe an immuno-dot blot assay for the inhibition profiling of HKs using the anti-N3-phosphohistidine antibody. This simple method promises reliable detection of HK activity, and it is likely applicable in high-throughput screening of HK inhibitors. |
| Databáze: | OpenAIRE |
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