Community dynamics of methanotrophic bacteria during composting of organic matter
| Autor: | Willy Verstraete, Dirk Halet, Nico Boon |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Electrophoresis
Time Factors Population Bioengineering engineering.material Biology complex mixtures Applied Microbiology and Biotechnology Polymerase Chain Reaction Microbiology Soil RNA Ribosomal 16S Environmental Microbiology Cluster Analysis Food science education Soil Microbiology education.field_of_study Models Statistical Bacteria Compost Reverse Transcriptase Polymerase Chain Reaction Thermophile fungi 16S ribosomal RNA biology.organism_classification Microbial population biology engineering Temperature gradient gel electrophoresis Mesophile Biotechnology |
| Zdroj: | Journal of bioscience and bioengineering. 101(4) |
| ISSN: | 1389-1723 |
| Popis: | In this study, we describe the effects of composting on the diversity, abundance and activity of the methanotrophic community present in the compost. Composting was allowed to proceed for 10 weeks in an in-vessel reactor. Self-heating capacity (Rottegrad) indicated that compost maturity was reached after 4 weeks. After 6 weeks, a second thermophilic phase was induced by manually increasing temperature to investigate whether or not the methanotrophs shifted back to the thermophilic population. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) with primers specific for type I methanotrophs of 16S rDNA and 16S rRNA were used to characterize the composition of the microbial community. Cluster and diversity analyses of RNA DGGE patterns were more sensitive than those of DNA DGGE patterns, and revealed that mesophilic and thermophilic methanotrophic communities could be differentiated. Moreover, it was seen that the diversity of the community was low during the thermophilic phase and increased during the final maturation phase. Real-time PCR analysis was also performed on the DNA and RNA extracts and showed no changes in the abundance of type I methanotrophs during the composting process (10 9 DNA copies/g compost). However, RNA-related activity did change, with the lowest activity (10 7 cDNA copies/g compost) observed during the thermophilic phase, subsequently increasing to its maximum value (10 9 cDNA copies/g compost), and finally decreasing during the maturation phase. This study confirmed the population dynamics, as seen for general groups such as bacteria and fungi during composting, for a very specific and sensitive group of bacteria, it is the type I methanotrophs. |
| Databáze: | OpenAIRE |
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