Development of surgery during the last 50 years
| Autor: | Henry Johansson |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Endogenous erythropoietin End stage renal disease medicine Humans Anesthesia Anesthetics Asepsis Sweden Novel Erythropoiesis Stimulating Protein Transplantation medicine.diagnostic_test biology business.industry History 19th Century General Medicine Venous blood History 20th Century Anti-Bacterial Agents Surgery Erythropoietin General Surgery Immunoassay biology.protein business Neuraminidase Immunosuppressive Agents medicine.drug |
| Zdroj: | Upsala Journal of Medical Sciences. 108:79-85 |
| ISSN: | 2000-1967 0300-9734 |
| Popis: | Doping with darbepoetin-α, also termed novel erythropoiesis stimulating protein (NESP), a hypersialylated, very effective analogue of erythropoietin, is a serious threat in sport. We report here on a new principle for the detection of darbepoetin-α in serum based upon increase in immunoactivity after desialylation with neuraminidase. The method is evaluated on sera from patients taken 2-14 days after last injection of darbepoetin-α.Thirty-two venous blood samples and 3 capillary samples taken from finger tips were obtained from 13 patients with end stage renal disease treated with intravenous or subcutaneous injections of Aranesp, 0.45 to 2.60 μg*kg-1. Blood samples from 37 individuals with endogenous erythropoietin were used as controls. The sera were diluted 1:2 with acetate buffer pH 5.6 with or without neuraminidase and incubated at 37°C for 1 or 24 h before immunoassay. The erythropoietin immunoactivity in serum volumes of 12.5-50 μL was measured with ELISA-kits from R&D Systems Inc and medac Gmb... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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