Determination of Organic Impurities in Anthraquinone Color Additives D&C Violet No. 2 and D&C Green No. 6 by Ultra-High Performance Liquid Chromatography
| Autor: | H H Wendy Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pharmacology
Detection limit Chromatography 010405 organic chemistry Calibration curve 010401 analytical chemistry Extraction (chemistry) Anthraquinones 01 natural sciences Anthraquinone 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Column chromatography chemistry Impurity Environmental Chemistry Coloring Agents Acetonitrile Agronomy and Crop Science Ammonium acetate Chromatography High Pressure Liquid Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 100:230-235 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.16-0067 |
| Popis: | A new practical and time-saving ultra-high performance liquid chromatography (UHPLC) method has been developed for determining the organic impurities in the anthraquinone color additives D&C Violet No. 2 and D&C Green No. 6. The impurities determined are p-toluidine, 1-hydroxyanthraquinone, 1,4-dihydroxyanthraquinone, and two subsidiary colors. The newly developed UHPLC method uses a 1.7-μ particle size C-18 column, 0.1 M ammonium acetate and acetonitrile aseluents, and photodiode array detection. For the quantification of the impurities, six-point calibration curves were used with correlation coefficients that ranged from 0.9974 to 0.9998. Recoveries of impurities ranged from 99 to 104%. Relative standard deviations ranged from 0.81 to 4.29%. The limits of detection for the impurities ranged from 0.0067% to0.216%. Samples from sixteen batches of each color additive were analyzed, and the results favorably compared with the results obtained by gravity-elution column chromatography, thin-layer chromatography, and isooctane extraction. Unlike with those other methods, use of the UHPLC method permits all of the impurities to be determined in a single analysis, while also reducing the amount of organic waste and saving timeand labor. The method is expected to be implemented by the U.S. Food and Drug Administration for analysis of color additive samples submitted for batch certification. |
| Databáze: | OpenAIRE |
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