Dimethyl cardamonin inhibits lipopolysaccharide-induced inflammatory factors through blocking NF-κB p65 activation
| Autor: | Evangeline C. Amor, Hyeonseok Ko, Jong Wha Lee, Jin-Soo Park, I. K. Han, Hyun Ok Yang, Young-Joo Kim |
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| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide medicine.medical_treatment Interleukin-1beta Immunology Anti-Inflammatory Agents Nitric Oxide Synthase Type II Pharmacology Nitric Oxide Dinoprostone Nitric oxide Mice chemistry.chemical_compound Chalcones Blood serum medicine Animals Immunology and Allergy Phosphorylation Prostaglandin E2 Inflammation Mice Inbred ICR biology Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Transcription Factor RelA NF-κB Shock Septic Nitric oxide synthase Biochemistry chemistry Cyclooxygenase 2 biology.protein Tumor necrosis factor alpha Inflammation Mediators Prostaglandin E medicine.drug |
| Zdroj: | International Immunopharmacology. 10:1127-1134 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2010.06.017 |
| Popis: | This study has found that dimethyl cardamonin (2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone; DMC), a naturally occurring chalcone, showed potent anti-inflammatory effects in vitro and in vivo. In a cellular model of inflammation, DMC inhibited production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) and attenuated expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), IL-1 beta, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). DMC prevented nuclear translocation of the nuclear factor-kappaB (NF-kappaB) p65 subunit by reducing inhibitor of kappaB alpha (I-kappaB alpha) phosphorylation and degradation, which resulted in a suppression of NF-kappaB activities for its target genes. In a mouse model of endotoxin shock, the intraperitoneal injection (i.p.) of DMC (1-50mg/kg) suppressed TNF-alpha, IL-6 and IL-1 beta secretion in LPS-induced mouse blood serum. These results suggest that DMC exerts anti-inflammatory effects through blocking NF-kappaB activation, therefore, DMC may act as an effective therapeutic strategy against a variety of inflammatory diseases. |
| Databáze: | OpenAIRE |
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