Effect of Physicochemical Surface Modifications on Bovine Serum Albumin Adsorption to Tetragonal Zirconia Polycrystal in vitro Through the change of the Zeta Potential
| Autor: | Koji Tanabe, Toru Sato, Tadashi Miura, Shuichiro Yamashita, Nahoko Miyake, Ryuichi Hisanaga, Masao Yoshinari |
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| Rok vydání: | 2016 |
| Předmět: |
Polymers
Surface Properties General Chemical Engineering Static Electricity Nanotechnology 02 engineering and technology law.invention 03 medical and health sciences 0302 clinical medicine Adsorption Magazine law Zeta potential Ultraviolet light Animals Bovine serum albumin biology Chemistry Physical Chemistry Serum Albumin Bovine 030206 dentistry General Medicine General Chemistry 021001 nanoscience & nanotechnology Electrostatics Chemical engineering biology.protein Surface modification Cattle Zirconium 0210 nano-technology Protein adsorption |
| Zdroj: | Journal of Oleo Science. 65:1003-1010 |
| ISSN: | 1347-3352 1345-8957 |
| DOI: | 10.5650/jos.ess16053 |
| Popis: | The technology of physicochemical surface modification is available for enhancing the bioactivity and osseointegration capability of tetragonal zirconia polycrystal (TZP). Hydrophobicity index and electrical charge play important roles in protein adsorption. We previously studied the mechanism underlying the adsorption of bovine serum albumin (BSA) on the surfaces of dental materials and hydroxyapatite in vitro. The aim of the present study was to clarify the correlation among the adsorption of BSA to TZP and physicochemically modified TZP surfaces and the zeta potential of BSA and TZP. We used TZP that was sintered at 1350°C for 2 h in air because this kind of TZP is widely applied in the field of dentistry. Surface physicochemistry was modified with ultraviolet light (UV) and atmospheric-pressure plasma treatment. The zeta potentials were measured with ELSZ-1000 and ELSZ-2000 analyzers (Otsuka Electronics, Hirakata, Japan). All experiments were conducted in 10 mM NaCl (pH 7.0). The zeta potentials of as-received TZP and BSA were negative, but those of UV- and plasma-treated TZP were positive. The reason the zeta potentials of TZP changed positive by physicochemical modification is due to an increase in the amount of basic hydroxyl groups. The zeta potentials of UV- and plasma-treated TZP after BSA adsorption were negative. These results suggested that electrostatic interactions play an important role in BSA adsorption to TZP and modified TZP surfaces, so that this modified surface may control the adsorption of protein. |
| Databáze: | OpenAIRE |
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