Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents
| Autor: | Choon-Hong Tan, Song Lin Chua, Liang Yang, Roger W. Beuerman, Yang Liu, Michael Givskov, Mingjun Yuan, Thomas E. Nielsen, Daniela I. Drautz-Moses, Stephan C. Schuster, May Margarette Santillan Salido, Joey Kuok Hoong Yam, Thet Tun Aung |
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| Přispěvatelé: | School of Biological Sciences, Lee Kong Chian School of Medicine (LKCMedicine), Singapore Centre for Environmental Life Sciences Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.drug_class 030106 microbiology Antibiotics Resistance cisplatin medicine.disease_cause biofilm Type three secretion system Microbiology resistance lcsh:QD241-441 03 medical and health sciences Antibiotic resistance lcsh:Organic chemistry In vivo type III secretion medicine lcsh:Science Cisplatin Pseudomonas aeruginosa Chemistry Biofilm Organic Chemistry Antimicrobial Science::Biological sciences [DRNTU] 030104 developmental biology Type III secretion lcsh:Q medicine.drug |
| Zdroj: | Beilstein Journal of Organic Chemistry, Vol 14, Iss 1, Pp 3059-3069 (2018) Yuan, M, Chua, S L, Liu, Y, Drautz-Moses, D I, Hoong Yam, J K, Aung, T T, Beuerman, R W, Santillan Salido, M M, Schuster, S C, Tan, C H, Givskov, M, Yang, L & Nielsen, T E 2018, ' Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents ', Beilstein Journal of Organic Chemistry, vol. 14, pp. 3059-3069 . https://doi.org/10.3762/bjoc.14.284 |
| ISSN: | 1860-5397 |
| DOI: | 10.3762/bjoc.14.284 |
| Popis: | Antibiotic resistance threatens effective treatment of microbial infections globally. This situation has spurred the hunt for new antimicrobial compounds in both academia and the pharmaceutical industry. Here, we report how the widely used antitumor drug cisplatin may be repurposed as an effective antimicrobial against the nosocomial pathogen Pseudomonas aeruginosa. Cisplatin was found to effectively kill strains of P. aeruginosa. In such experiments, transcriptomic profiling showed upregulation of the recA gene, which is known to be important for DNA repair, implicating that cisplatin could interfere with DNA replication in P. aeruginosa. Cisplatin treatment significantly repressed the type III secretion system (T3SS), which is important for the secretion of exotoxins. Furthermore, cisplatin was also demonstrated to eradicate in vitro biofilms and in vivo biofilms in a murine keratitis model. This showed that cisplatin could be effectively used to eradicate biofilm infections which were otherwise difficult to be treated by conventional antibiotics. Although cisplatin is highly toxic for humans upon systemic exposure, a low toxicity was demonstrated with topical treatment. This indicated that higher-than-minimal inhibitory concentration (MIC) doses of cisplatin could be topically applied to treat persistent and recalcitrant P. aeruginosa infections. |
| Databáze: | OpenAIRE |
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