Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

Autor: Alejandro Navas, Fátima Sofía Magaña-Guerrero, Alfredo Domínguez-López, César Chávez-García, Graciela Partido, Enrique O Graue-Hernandez, Francisco Javier Sánchez-García, Yonathan Garfias
Rok vydání: 2018
Předmět:
0301 basic medicine
Stromal cell
Neutrophils
Inflammation
Mesenchymal Stem Cell Transplantation
Regenerative medicine
Corneal Diseases
Proinflammatory cytokine
Cornea
03 medical and health sciences
Translational Research Articles and Reviews
Tissue Engineering and Regenerative Medicine
Burns
Chemical
medicine
Animals
Humans
Amnion
Myofibroblasts
Cells
Cultured
Intraocular Pressure
Cell Proliferation
business.industry
Corneal repair
Mesenchymal stem cell
NETs
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
General Medicine
Neutrophil extracellular traps
hAM‐MSC
Transplantation
Disease Models
Animal
030104 developmental biology
α‐SMA myofibroblasts
Microscopy
Fluorescence
Culture Media
Conditioned
Cancer research
Rabbits
Stem cell
medicine.symptom
business
Tomography
Optical Coherence
Developmental Biology
Zdroj: Stem Cells Translational Medicine
ISSN: 2157-6580
2157-6564
Popis: Acute ocular chemical burns are ophthalmic emergencies requiring immediate diagnosis and treatment as they may lead to permanent impairment of vision. The clinical manifestations of such burns are produced by exacerbated innate immune response via the infiltration of inflammatory cells and activation of stromal fibroblasts. New therapies are emerging that are dedicated to repair mechanisms that improve the ocular surface after damage; for example, transplantation of stem cells (SC) has been successfully reported for this purpose. The pursuit of easily accessible, noninvasive procedures to obtain SC has led researchers to focus on human tissues such as amniotic membrane. Human amniotic mesenchymal SC (hAM-MSC) inhibits proinflammatory and fibrotic processes in different diseases. hAM-MSC expresses low levels of classical MHC-I and they do not express MHC-II, making them suitable for regenerative medicine. The aim of this study was to evaluate the effect of intracameral injection of hAM-MSC on the clinical manifestations, the infiltration of inflammatory cells, and the activation of stromal fibroblasts in a corneal alkali-burn model. We also determined the in vitro effect of hAM-MSC conditioned medium (CM) on α-SMA+ human limbal myofibroblast (HLM) frequency and on release of neutrophil extracellular traps (NETs). Our results show that intracameral hAM-MSC injection reduces neovascularization, opacity, stromal inflammatory cell infiltrate, and stromal α-SMA+ cells in our model. Moreover, in in vitro assays, CM from hAM-MSC decreased the quantity of α-SMA+ HLM and the release of NETs. These results suggest that intracameral hAM-MSC injection induces an anti-inflammatory and anti-fibrotic environment that promotes corneal wound healing.
Databáze: OpenAIRE
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