Localization of C-X-C and C-C chemokines to renal tubular epithelial cells in human kidney transplants is not confined to acute cellular rejection
| Autor: | J S Sibbring, R. A. Sells, Stephen E. Christmas, Ajay Sharma, I.W. McDicken |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Graft Rejection Male Chemokine Pathology medicine.medical_specialty Urinary system Immunology chemistry.chemical_compound Biopsy medicine Humans Immunology and Allergy Macrophage Chemokine CCL4 Chemokine CCL2 Transplantation Creatinine Kidney Staining and Labeling biology medicine.diagnostic_test Monocyte Interleukin-8 Epithelial Cells Macrophage Inflammatory Proteins Middle Aged Kidney Transplantation Kidney Tubules medicine.anatomical_structure chemistry Chemokines CC Acute Disease biology.protein Immunohistochemistry Female Kidney Diseases Chemokines CXC |
| Zdroj: | Transplant Immunology. 6:203-208 |
| ISSN: | 0966-3274 |
| Popis: | Chemokines are important mediators of leucocyte chemoattraction to inflammatory sites. Previous work has shown that the expression of some chemokines is upregulated during renal transplant rejection. The objectives of the present study were to determine whether chemokine expression is increased during renal transplant rejection. Immunohistochemistry was used to localize the C-X-C (alpha) chemokine interleukin-8 (IL-8) and the C-C (beta) chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1beta (MIP-1beta) in 30 needle biopsies of human kidney transplants taken for diagnosis of renal dysfunction. Urine samples from transplant patients taken immediately prior to biopsy were assayed for chemokine content using enzyme-linked immunosorbent assays (ELISAs). Results from groups of patients having different clinicopathological diagnoses were then compared. All three chemokines were detected in most renal transplant biopsies showing acute cellular rejection but, although infiltrating leucocytes were often positive, staining was predominantly localized to renal tubular epithelium. Staining for MCP-1 was generally weaker than for the other chemokines, and collecting tubules were usually stained more strongly than proximal convoluted tubules. Tubular epithelial staining was also found in biopsies from patients without signs of acute cellular rejection. There were significantly higher amounts of IL-8 in the urine of patients with acute cellular rejection, even when patients with urinary tract infections were excluded, but mean titres of urinary MIP-1beta did not differ between patient groups. This was also found when titres were normalized for urine volume and creatinine levels. Production of IL-8, MCP-1 and MIP-1beta is not confined to kidney transplants showing acute cellular rejection, and may be a relatively nonspecific response of tubular epithelial cells to renal damage. |
| Databáze: | OpenAIRE |
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