Immuno-electron microscopy of atrial natriuretic factor secretory pathways in atria and ventricles of control and cardiomyopathic hamsters with heart failure
| Autor: | Jacques Genest, Raul Garcia, Gaétan Thibault, Marc Cantin, Gaëtan Jasmin, M. Ballak, H Haile-Meskel |
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| Rok vydání: | 1990 |
| Předmět: |
Cardiomyopathy
Dilated medicine.medical_specialty Histology Heart Ventricles Immunoelectron microscopy Biology Exocytosis Pathology and Forensic Medicine symbols.namesake Atrial natriuretic peptide Cricetinae Internal medicine medicine Animals Secretion Heart Atria cardiovascular diseases Heart Failure Mesocricetus Myocardium Antibodies Monoclonal Heart Cell Biology Golgi apparatus NPR1 medicine.disease Immunohistochemistry Secretory Vesicle NPR2 Microscopy Electron Endocrinology Heart failure cardiovascular system symbols Atrial Natriuretic Factor |
| Zdroj: | Cell and Tissue Research. 261:313-322 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/bf00318672 |
| Popis: | The secretory pathways of atrial natriuretic factor have been investigated in atrial and ventricular cardiocytes of control and cardiomyopathic Syrian hamsters in severe congestive heart failure with four antibodies: a monoclonal antibody (2H2) against rat synthetic atrial natriuretic factor (101-126), which is directed against region 101-103 of rat atrial natriuretic factor (99-126), and polyclonal, affinity-purified antibodies produced in rabbits against synthetic C-terminal atrial natriuretic factor (101-126), synthetic N-terminal atrial natriuretic factor (11-37) or the putative cleavage site of atrial natriuretic factor (98-99): atrial natriuretic factor (94-103). Application of the immunogold technique on thin frozen sections (immunocryoultramicrotomy) revealed an identical picture with the four antibodies. In atria of both control and cardiomyopathic hamsters where atrial natriuretic factor secretion is regulated, the atrial natriuretic factor propeptide travels, uncleaved, from the Golgi complex to immature and mature secretory granules. In ventricles of control hamsters, where secretion is constitutive, the atrial natriuretic factor propeptide travels from the Golgi complex to secretory vesicles. In the ventricles of hamsters with severe congestive heart failure, the Golgi complex is larger, secretory vesicles more abundant and a few secretory granules are present in approximately 20% of cardiocytes. Here again, the peptide travels uncleaved in all these pathways. These results reveal the pathways of secretion of atrial natriuretic factor in atrial and ventricular cardiocytes and indicate that the propeptide is not cleaved intracellularly. |
| Databáze: | OpenAIRE |
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