6-Shogaol, an active compound of ginger, protects dopaminergic neurons in Parkinson's disease models via anti-neuroinflammation
Autor: | Sang Keun Ha, Sun Yeou Kim, Yongkon Park, Gunhyuk Park, Hyo Geun Kim, Myung Sook Oh, Mi Sun Ju |
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Rok vydání: | 2013 |
Předmět: |
Male
Parkinson's disease Stereochemistry Catechols Ginger Pharmacology Rats Sprague-Dawley Mice chemistry.chemical_compound Parkinsonian Disorders Mesencephalon Pregnancy Animals Medicine Pharmacology (medical) Cells Cultured Plant Extracts business.industry Dopaminergic Neurons Dopaminergic General Medicine Shogaol medicine.disease Rats Mice Inbred C57BL Disease Models Animal Anti neuroinflammation Neuroprotective Agents chemistry Active compound Original Article Female business |
Zdroj: | Acta Pharmacologica Sinica. 34:1131-1139 |
ISSN: | 1745-7254 1671-4083 |
Popis: | 6-Shogaol [1-(4-hydroxy-methoxyphenyl)-4-decen-one], a pungent compound isolated from ginger, has shown various neurobiological and anti-inflammatory effects. The aim of this study was to examine the effects of 6-shogaol on neuroinflammatory-induced damage of dopaminergic (DA) neurons in Parkinson's disease (PD) models.Cultured rat mesencephalic cells were treated with 6-shogaol (0.001 and 0.01 μmol/L) for 1 h, then with MPP(+)(10 μmol/L) for another 23 h. The levels of TNF-α and NO in medium were analyzed spectrophotometrically. C57/BL mice were administered 6-shogaol (10 mg·kg(-1)·d(-1), po) for 3 d, and then MPTP (30 mg/kg, ip) for 5 d. Seven days after the last MPTP injection, behavioral testings were performed. The levels of tyrosine hydroxylase (TH) and macrophage antigen (MAC)-1 were determined with immunohistochemistry. The expression of iNOS and COX-2 was measured using RT PCR.In MPP(+)-treated rat mesencephalic cultures, 6-shogaol significantly increased the number of TH-IR neurons and suppressed TNF-α and NO levels. In C57/BL mice, treatment with 6-shogaol reversed MPTP-induced changes in motor coordination and bradykinesia. Furthermore, 6-shogaol reversed MPTP-induced reductions in TH-positive cell number in the substantia nigra pars compacta (SNpc) and TH-IR fiber intensity in stratum (ST). Moreover, 6-shogaol significantly inhibited the MPTP-induced microglial activation and increases in the levels of TNF-α, NO, iNOS, and COX-2 in both SNpc and ST.6-Shogaol exerts neuroprotective effects on DA neurons in in vitro and in vivo PD models. |
Databáze: | OpenAIRE |
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