Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
| Autor: | Shao-Nong Chen, Qingfei Fan, J. Brent Friesen, Guido F. Pauli, Yang Liu, Daniel Kulakowski, Qishi Song |
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| Rok vydání: | 2018 |
| Předmět: |
Vitex negundo
Countercurrent exchange medicine.medical_treatment 01 natural sciences Biochemistry Article Analytical Chemistry Steroid Vitex medicine Humans Countercurrent Distribution Progesterone Solvent system Chromatography biology Estriol 010405 organic chemistry Chemistry fungi 010401 analytical chemistry Organic Chemistry General Medicine biology.organism_classification 0104 chemical sciences Steroid hormone Hormone Plant Sources |
| Zdroj: | Journal of Chromatography A. 1553:108-115 |
| ISSN: | 0021-9673 |
| Popis: | Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds. |
| Databáze: | OpenAIRE |
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