Cytotoxic activity of pierisin, from the cabbage butterfly, Pieris rapae, in various human cancer cell lines
| Autor: | Shoji Fukushima, Takuo Kono, Takashi Sugimura, Masahiko Watanabe, Taketoshi Kishimoto, Keiji Wakabayashi, Kotaro Koyama |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Apoptosis Pieris rapae Umbilical vein HeLa Tumor Cells Cultured medicine Animals Humans Cytotoxic T cell Cytotoxicity ADP Ribose Transferases biology Cytotoxins Cancer medicine.disease biology.organism_classification Molecular biology Oncology Cell culture Immunology Insect Proteins Drug Screening Assays Antitumor Butterflies HeLa Cells |
| Zdroj: | Cancer Letters. 137:75-81 |
| ISSN: | 0304-3835 |
| Popis: | Pierisin, a protein purified from pupae of the cabbage butterfly, Pieris rapae , exhibits cytotoxic effects against the human gastric cancer TMK-1 cell line, inducing apoptosis. The present study was performed to determine whether pierisin might exert a similar influence on nine other human cancer cell lines and human umbilical vein endothelial cells (HUVECs). Pierisin showed cytotoxic effects in all the human cells tested, with IC 50 values ranging from 0.043 ng/ml to 150 ng/ml. Among the target cells, the cervical carcinoma cell line, HeLa, was the most sensitive to pierisin, showing a 1000-fold less IC 50 value than that of HUVECs. While pierisin clearly induced apoptotic cell death in most cancer cell lines and HUVECs, the pathway appeared to be probably different from that involving anti-Fas, TNF-α and p53. Pierisin may thus be a promising new candidate for cancer therapy. |
| Databáze: | OpenAIRE |
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