Impacts of different promoters on the mammalian one-hybrid assay for detecting nuclear receptor agonists
| Autor: | Hua Zhang, Zhihui Zheng, Xinhua Lu, Shuyi Si, Jiangong He, Guo-Ping Lv, Bao-Hua Zhao |
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| Rok vydání: | 2009 |
| Předmět: |
Hydrocarbons
Fluorinated TATA box High-throughput screening Genetic Vectors Peroxisome Proliferator-Activated Receptors Chenodeoxycholic Acid Ligands Biochemistry Sensitivity and Specificity Analytical Chemistry Rosiglitazone Transactivation Mice Structure-Activity Relationship Two-Hybrid System Techniques High-Throughput Screening Assays Drug Discovery Animals Humans Promoter Regions Genetic Transcription factor Cells Cultured Reporter gene Sulfonamides Pioglitazone Chemistry Promoter Molecular biology Cell biology Pyrimidines Nuclear receptor NIH 3T3 Cells Thiazolidinediones Bezafibrate HeLa Cells |
| Zdroj: | Analytical and bioanalytical chemistry. 396(5) |
| ISSN: | 1618-2650 |
| Popis: | Nuclear receptors are a superfamily of ligand-activated transcription factors that play key roles in many biological processes, and have become one class of the most important targets in drug discovery. Mammalian one-hybrid system has been used to develop a cell-based functional transactivation high-throughput screening (HTS) assay for detecting nuclear receptors ligands. In the present study, we proved that different promoters used in the reporter vector had significant different impacts on the performance of HTS assays. The assay using the SV40 promoter in the reporter vector showed the characteristics of much higher signal/noise ratios, acceptable Z′ factors (>0.6), low coefficient variation ( |
| Databáze: | OpenAIRE |
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