Nuclear lamins
| Autor: | Philippe Collas, Eivind G. Lund |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Genetics
animal structures integumentary system Transcription Genetic Stem Cells Research Cellular differentiation Promoter Cell Biology Biology Lamin Type A Chromatin Cell biology Adipose Tissue embryonic structures Gene expression Humans Nuclear lamina Promoter Regions Genetic ChIA-PET Lamin Progenitor |
| Zdroj: | Nucleus. 4:424-430 |
| ISSN: | 1949-1042 1949-1034 |
| DOI: | 10.4161/nucl.26865 |
| Popis: | The nuclear lamina is implicated in the organization of the eukaryotic nucleus. Association of nuclear lamins with the genome occurs through large chromatin domains including mostly, but not exclusively, repressed genes. How lamin interactions with regulatory elements modulate gene expression in different cellular contexts is unknown. We show here that in human adipose tissue stem cells, lamin A/C interacts with distinct spatially restricted subpromoter regions, both within and outside peripheral and intra-nuclear lamin-rich domains. These localized interactions are associated with distinct transcriptional outcomes in a manner dependent on local chromatin modifications. Down-regulation of lamin A/C leads to dissociation of lamin A/C from promoters and remodels repressive and permissive histone modifications by enhancing transcriptional permissiveness, but is not sufficient to elicit gene activation. Adipogenic differentiation resets a large number of lamin-genome associations globally and at subpromoter levels and redefines associated transcription outputs. We propose that lamin A/C acts as a modulator of local gene expression outcome through interaction with adjustable sites on promoters, and that these position-dependent transcriptional readouts may be reset upon differentiation. |
| Databáze: | OpenAIRE |
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