Identification of Major Rhizobacterial Taxa Affected by a Glyphosate-Tolerant Soybean Line via Shotgun Metagenomic Approach
| Autor: | Jin-Liang Qi, Xiao-Mei Hua, Cheng-Yi Tang, Fan-Fan Meng, Gui-Hua Lu, Yan-Jun Pang, Li Liang, Zhongling Wen, Mei-Hang Du, Yong-Hua Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
plant growth-promoting rhizobacteria lcsh:QH426-470 glyphosate-tolerant soybean Rhizobacteria Bradyrhizobium Article soil 03 medical and health sciences Botany Genetics Bacterial phyla Genetics (clinical) Rhizosphere biology food and beverages shotgun metagenomic approach biology.organism_classification 16S ribosomal RNA lcsh:Genetics 030104 developmental biology Metagenomics Janthinobacterium Stenotrophomonas 16S rRNA gene rhizosphere |
| Zdroj: | Genes; Volume 9; Issue 4; Pages: 214 Genes, Vol 9, Iss 4, p 214 (2018) Genes |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes9040214 |
| Popis: | The worldwide commercial cultivation of transgenic crops, including glyphosate-tolerant (GT) soybeans, has increased widely during the past 20 years. However, it is accompanied with a growing concern about potential effects of transgenic crops on the soil microbial communities, especially on rhizosphere bacterial communities. Our previous study found that the GT soybean line NZL06-698 (N698) significantly affected rhizosphere bacteria, including some unidentified taxa, through 16S rRNA gene (16S rDNA) V4 region amplicon deep sequencing via Illumina MiSeq. In this study, we performed 16S rDNA V5–V7 region amplicon deep sequencing via Illumina MiSeq and shotgun metagenomic approaches to identify those major taxa. Results of these processes revealed that the species richness and evenness increased in the rhizosphere bacterial communities of N698, the beta diversity of the rhizosphere bacterial communities of N698 was affected, and that certain dominant bacterial phyla and genera were related to N698 compared with its control cultivar Mengdou12. Consistent with our previous findings, this study showed that N698 affects the rhizosphere bacterial communities. In specific, N698 negatively affects Rahnella, Janthinobacterium, Stenotrophomonas, Sphingomonas and Luteibacter while positively affecting Arthrobacter, Bradyrhizobium, Ramlibacter and Nitrospira. |
| Databáze: | OpenAIRE |
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