Complete genome of a unicellular parasite (Antonospora locustae) and transcriptional interactions with its host locust
| Autor: | Feng Wang, Ting Liu, Yue Song, Runlin Z. Ma, Shuang Hua, Zhenzhen Xing, Runting Li, Limeng Zhang, Zihan Zhang, Linqing Wang, Xingke Gao, Rui Huang, Xiaoning Nie, Long Zhang, Fangyuan Nie, Longxin Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Host–pathogen interaction
Fat Body Peroxisome Proliferator-Activated Receptors Grasshoppers Biology Genome 03 medical and health sciences 0302 clinical medicine Genome Size parasitic diseases Animals Parasite hosting Microbe-Niche Interactions: Host adaptation genome Genome size Gene Phylogeny 030304 developmental biology Genetics Base Composition 0303 health sciences Host Microbial Interactions Whole Genome Sequencing Monophenol Monooxygenase Gene Expression Profiling fungi High-Throughput Nucleotide Sequencing Molecular Sequence Annotation General Medicine biology.organism_classification locust Gene Expression Regulation Microsporidia Insect Proteins Chromosomes Fungal host–pathogen interaction transcription 030217 neurology & neurosurgery GC-content Locust Research Article |
| Zdroj: | Microbial Genomics |
| ISSN: | 2057-5858 |
| DOI: | 10.1099/mgen.0.000421 |
| Popis: | Microsporidia are a large group of unicellular parasites that infect insects and mammals. The simpler life cycle of microsporidia in insects provides a model system for understanding their evolution and molecular interactions with their hosts. However, no complete genome is available for insect-parasitic microsporidian species. The complete genome ofAntonospora locustae, a microsporidian parasite that obligately infects insects, is reported here. The genome size ofA. locustaeis 3 170 203 nucleotides, composed of 17 chromosomes onto which a total of 1857 annotated genes have been mapped and detailed. A unique feature of theA. locustaegenome is the presence of an ultra-low GC region of approximately 25 kb on 16 of the 17 chromosomes, in which the average GC content is only 20 %. Transcription profiling indicated that the ultra-low GC region of the parasite could be associated with differential regulation of host defences in the fat body to promote the parasite’s survival and propagation. Phylogenetic gene analysis showed thatA. locustae, and the microsporidian family in general, is likely at an evolutionarily transitional position between prokaryotes and eukaryotes, and that it evolved independently. Transcriptomic analysis showed thatA. locustaecan systematically inhibit the locust phenoloxidase PPO, TCA and glyoxylate cycles, and PPAR pathways to escape melanization, and can activate host energy transfer pathways to support its reproduction in the fat body, which is an insect energy-producing organ. Our study provides a platform and model for studies of the molecular mechanisms of microsporidium–host interactions in an energy-producing organ and for understanding the evolution of microsporidia. |
| Databáze: | OpenAIRE |
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