Next-generation sequencing characterization of HLA in multi-generation families of Kuwaiti descent
Autor: | Reem Ameen, Medhat Askar, Salem Al Shemmari |
---|---|
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Genotype Immunology Population Human leukocyte antigen Biology DNA sequencing Cohort Studies 03 medical and health sciences 0302 clinical medicine Gene Frequency HLA Antigens Immunology and Allergy Humans Family Typing Registries Allele education Alleles Genetics education.field_of_study Hla haplotypes Haplotype High-Throughput Nucleotide Sequencing General Medicine Tissue Donors United States Pedigree Black or African American 030104 developmental biology Haplotypes Kuwait Cohort 030215 immunology |
Zdroj: | Human immunology. 79(3) |
ISSN: | 1879-1166 |
Popis: | The frequency of HLA genes in one population may not accurately represent frequencies in other populations. In this study, we characterized extended human leukocyte antigen (HLA) haplotypes in several families of Kuwaiti descent by high-resolution typing using next-generation technology. A total 81 members (including patients and related donors) from 21 families were enrolled. No haplotypes were shared among multiple families. Of 77 haplotypes identified, 23 were not listed in the HaploStats database. Two haplotypes were most common in African Americans, six in Asian Pacific Islanders, three in Caucasians, three in Hispanics, and three in Native Americans. The remaining identified haplotypes were not among the most common 200 HLA haplotypes in any of the five major populations. This cohort had 202 (19%) unique alleles, including 20 rare alleles, 16 very rare alleles, and 2 novel ones. Furthermore, no frequency data were available for 30% (23/77) of the observed haplotypes, and 6% (3/49) of B ∼ C blocks identified were not available in the HaploStats database. Kuwaiti individuals carry unique HLA haplotypes that are not shared by the majority of individuals historically reported to the US National Marrow Donor Program registry. |
Databáze: | OpenAIRE |
Externí odkaz: |