The Role of Forkhead Box Q1 Transcription Factor in Ovarian Epithelial Carcinomas
| Autor: | Ie Ming Shih, Tian Li Wang, Min Gao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Cell
Gene Expression Carcinoma Ovarian Epithelial migration ovarian cancer FOXQ1 survival invasion lcsh:Chemistry 0302 clinical medicine Cell Movement Neoplasms Glandular and Epithelial lcsh:QH301-705.5 Spectroscopy Tumor Stem Cell Assay Ovarian Neoplasms 0303 health sciences Forkhead Transcription Factors General Medicine Cell cycle 3. Good health Computer Science Applications Cell biology Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure 030220 oncology & carcinogenesis Gene Knockdown Techniques Female Epithelial-Mesenchymal Transition Biology Catalysis Article Inorganic Chemistry 03 medical and health sciences Downregulation and upregulation Forkhead box Q1 Cell Line Tumor medicine Gene silencing Humans Physical and Theoretical Chemistry Molecular Biology Transcription factor 030304 developmental biology Cell Proliferation Cell growth Gene Expression Profiling Organic Chemistry Cell Cycle Checkpoints medicine.disease lcsh:Biology (General) lcsh:QD1-999 Ovarian cancer |
| Zdroj: | International Journal of Molecular Sciences, Vol 13, Iss 11, Pp 13881-13893 (2012) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 13; Issue 11; Pages: 13881-13893 |
| ISSN: | 1422-0067 |
| Popis: | The role of the forkhead box Q1 (FOXQ1) transcription factor in cancer pathogenesis has recently emerged. Overexpression of FOXQ1 has been found in a variety of human cancers, and its upregulation has been associated with poor prognosis in colorectal, breast, and non-small cell lung carcinomas. However, the molecular mechanism underlying how FOXQ1 contributes to ovarian epithelial carcinomas remains unclear. To this end, we analyzed gene expression levels in ovarian cancer tissues and cell lines and demonstrated a higher expression level of FOXQ1 in epithelial ovarian cancer cells than that in normal epithelial cells. We then used a human ovarian cancer cell line, SKOV3, which expressed a higher level of FOXQ1, as a cell model to investigate the biological effects of FOXQ1 by using RNA interference. Silencing of FOXQ1 expression using a shRNA knockdown approach affected the expression of several cell cycle regulators, leading to suppressed cell proliferation, reduced cell motility/invasion, and upregulation of epithelial cell markers and the downregulation of mesenchymal cell markers. Taken together, these results suggest that FOXQ1 expression is essential to maintain cell proliferation, motility/invasion, and epithelial-mesenchymal transition phenotypes in ovarian cancer cells. |
| Databáze: | OpenAIRE |
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