The role of vimentin as a methylation biomarker for early diagnosis of cervical cancer
| Autor: | Chang-Hwan Kim, Jin Shin, Lisha Yi, Dongjun Jeong, Samil Jung, TaeJeong Oh, Jinsun Kim, Sungwhan An, Myeong-Sok Lee, Chang-Jin Kim |
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| Rok vydání: | 2011 |
| Předmět: |
Uterine Cervical Neoplasms
Vimentin Cervical intraepithelial neoplasia Cell Line Tumor Biomarkers Tumor polycyclic compounds medicine Humans Gene silencing Epigenetics Promoter Regions Genetic Molecular Biology Early Detection of Cancer biology Gene Expression Profiling Promoter Articles DNA Neoplasm Cell Biology General Medicine Methylation DNA Methylation biochemical phenomena metabolism and nutrition Uterine Cervical Dysplasia bacterial infections and mycoses medicine.disease Molecular biology Gene Expression Regulation Neoplastic CpG site DNA methylation biology.protein Cancer research Female HeLa Cells |
| Zdroj: | Molecules and Cells. 31:405-411 |
| ISSN: | 0219-1032 1016-8478 |
| DOI: | 10.1007/s10059-011-0229-x |
| Popis: | Multiple cytosine guanine dinucleotides (CpG island) are found in the VIM promoter region. The levels of VIM promoter methylation and VIM gene expression were investigated in 7 cervical cancer cell lines and 50 human tissue samples with a distinctive degree of malignant trans-formation. While multiple CpG sites in the VIM promoter were highly methylated in CIN III and invasive carcinoma cells, they were rarely methylated in normal cells. Our result shows that methylation in the VIM promoter appears to start from CIN I and CIN II, relatively early stages of multistep carcinogenesis. This epigenetic alteration in VIM promoter suggests the availability as a biomarker for the early diagnosis and prevention of cervical cancer. We also show that hypermethylation in the VIM promoter is responsible for transcriptional silencing of the VIM gene in cervical cancer cells. In addition, our result shows that exogenous overexpression of the VIM gene in SiHa cervical cancer cells slightly activated cell proliferation and migration as shown in soft agar colony formation and migration assays. |
| Databáze: | OpenAIRE |
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