Immunological Characterization of Maize Starch Branching Enzymes

Autor:	Charles D. Boyer, Mary B. Fisher
Rok vydání:	1983
Předmět:	Antiserum chemistry.chemical_classification biology Physiology Chemistry Immunoprecipitation Starch Articles Plant Science Branching (polymer chemistry) Enzyme assay Endosperm chemistry.chemical_compound Enzyme Biochemistry Genetics Glycogen branching enzyme biology.protein
Zdroj:	Plant Physiology. 72:813-816
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.72.3.813
Popis:	Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate was formed when branching enzyme I was tested against branching enzyme IIa or IIb antiserum. Increasing amounts of antisera in the above combinations also failed to inhibit enzyme activity. Branching enzyme IIa antiserum cross-reacted and formed spurs with branching enzyme IIb when compared with branching enzyme IIa antigen. Comparison of branching enzyme IIb antiserum with branching enzyme IIa also resulted in an immunoprecipitate. Increasing levels of branching enzyme IIa antiserum inhibited branching enzyme IIb as did the reciprocal combination. The data indicated that branching enzymes IIa and IIb are immunologically similar while branching enzyme I is distinct. The data supports the classification of starch branching enzymes based on genetic, kinetic, and chromatographic properties.
Databáze:	OpenAIRE
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