Separation of reduced disaccharides derived from glycosaminoglycans by high-performance liquid chromatography
| Autor: | Joseph W. Pav, George Jia-Long Lee, Der-Wu Liu, Howard Tieckelmann |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Mucopolysaccharidosis VI
Chromatography Organic Chemistry Disaccharide General Medicine Heparan sulfate Heparin Biochemistry High-performance liquid chromatography Dermatan sulfate Chondroitinases and Chondroitin Lyases Analytical Chemistry carbohydrates (lipids) Glycosaminoglycan chemistry.chemical_compound Sodium borohydride chemistry medicine Humans Chondroitin Organic chemistry Oxidation-Reduction Chromatography High Pressure Liquid Glycosaminoglycans medicine.drug |
| Zdroj: | Journal of Chromatography A. 212:65-73 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)80547-6 |
| Popis: | A high-performance liquid chromatographic (HPLC) method for the analysis of reduced unsaturated disaccharides derived from enzymatic digestion followed by reduction with sodium borohydride of chondroitin sulfates, dermatan sulfate, heparan sulfate and heparin is described. This method is well suited for the HPLC analysis of glycosaminoglycans (GAGs) because the possibility of obtaining anomeric forms of unsaturated disaccharides is eliminated which provides a major advantage for quantitation. This procedure is more sensitive than existing HPLC methods for the determination of enzymatic degradation products from GAGs. In particular, the resolution of disaccharide products from heparan sulfate is improved after reduction. The applicability of this method for the determination of GAGs in biological sampales is demonstrated. |
| Databáze: | OpenAIRE |
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