IRF7 as an Immune-Related Prognostic Biomarker and Associated with Tumor Microenvironment in Low-Grade Glioma
| Autor: | Zhihua Cheng, Zhilin Guo, Shijun Peng, Guangyu Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
interferon regulatory factor 7
Tumor microenvironment business.industry medicine.medical_treatment International Journal of General Medicine General Medicine Immunotherapy medicine.disease survival analysis Immune system tumor-infiltrating immune cells glioma Glioma medicine Cancer research IRF7 business Gene CD8 Original Research Interferon regulatory factors |
| Zdroj: | International Journal of General Medicine |
| ISSN: | 1178-7074 |
| DOI: | 10.2147/ijgm.s324307 |
| Popis: | Purpose Tumor microenvironment (TME) affects the occurrence and progression of low-grade glioma (LGG). The aim of this study is to identify TME-related genes that influence prognosis in LGG patients and to explore their function and role in tumor immunity. Patients and Methods The TME components of LGG samples in the Cancer Genome Atlas (TCGA) database were identified by the ESTIMATE method, and differentially expressed genes (DEGs) with significant differences in immune scores and stromal scores were screened out. The core genes of DEGs were screened out by protein–protein interaction (PPI) network. Furthermore, immune-related target genes significantly correlated with prognosis were identified. Survival analysis and correlation analysis showed the correlation between target genes and clinical features and prognosis. The expression differences of target genes were verified by external database Chinese Glioma Genome Atlas (CGGA). CIBERSORT software identified the proportion of tumor-infiltrating immune cells (TICs) that were significantly related to target genes. Gene set enrichment analysis (GSEA) could enrich the main functions related to high and low expression of target genes. Results A total of 1567 DEGs were screened out from 529 LGG samples in the TCGA database, and 146 immune-related genes affecting prognosis were found. A total of 403 core genes were obtained from PPI network. The target gene interferon regulatory factor 7 (IRF7) was significantly associated with prognosis and clinical features of the tumor. The CGGA database verified the relationship between high and low expression groups of IRF7 and prognosis. GSEA indicated that IRF7 was mainly enriched in immune-related activities, significantly correlated with T cells CD8, macrophages M1, macrophages M2 and monocytes. Conclusion The IRF7 is involved in immune responses in TME of LGG, which in turn influenced tumor occurrence and progression. IRF7 can act as a potential biomarker for prognosis in patients with LGG and provide a target for tumor immunotherapy. Graphical Abstract |
| Databáze: | OpenAIRE |
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