Anatomy of zebrafish cerebellum and screen for mutations affecting its development
| Autor: | Takashi Shimizu, Yukiko Kimura, Young-Ki Bae, Koji Tanabe, Shuichi Kani, Shin-ichi Higashijima, Hideaki Nojima, Masahiko Hibi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cerebellum
Parallel fibers Molecular Sequence Data Purkinje cell Hindbrain Biology Deep cerebellar nuclei Neural circuits Animals Genetically Modified 03 medical and health sciences Glutamatergic 0302 clinical medicine Glutamates medicine Animals Mossy fibers Amino Acid Sequence Molecular Biology Zebrafish In Situ Hybridization gamma-Aminobutyric Acid DNA Primers 030304 developmental biology Eurydendroid cells Mutant screening 0303 health sciences Base Sequence Aldolase C Cell Biology Anatomy biology.organism_classification Granule cell Immunohistochemistry medicine.anatomical_structure nervous system Purkinje cells Climbing fibers Mutation Neuroglia Granule cells 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. 330:406-426 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2009.04.013 |
| Popis: | The cerebellum is important for the integration of sensory perception and motor control, but its structure has mostly been studied in mammals. Here, we describe the cell types and neural tracts of the adult zebrafish cerebellum using molecular markers and transgenic lines. Cerebellar neurons are categorized to two major groups: GABAergic and glutamatergic neurons. The Purkinje cells, which are GABAergic neurons, express parvalbumin7, carbonic anhydrase 8, and aldolase C like (zebrin II). The glutamatergic neurons are vglut1+ granule cells and vglut2high cells, which receive Purkinje cell inputs; some vglut2high cells are eurydendroid cells, which are equivalent to the mammalian deep cerebellar nuclei. We found olig2+ neurons in the adult cerebellum and ascertained that at least some of them are eurydendroid cells. We identified markers for climbing and mossy afferent fibers, efferent fibers, and parallel fibers from granule cells. Furthermore, we found that the cerebellum-like structures in the optic tectum and antero-dorsal hindbrain show similar Parvalbumin7 and Vglut1 expression profiles as the cerebellum. The differentiation of GABAergic and glutamatergic neurons begins 3 days post-fertilization (dpf), and layers are first detectable 5 dpf. Using anti-Parvalbumin7 and Vglut1 antibodies to label Purkinje cells and granule cell axons, respectively, we screened for mutations affecting cerebellar neuronal development and the formation of neural tracts. Our data provide a platform for future studies of zebrafish cerebellar development. |
| Databáze: | OpenAIRE |
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