Histologic study of the cellular events during rat mandibular distraction osteogenesis
| Autor: | Chikara Saito, Kimimitsu Oda, Rezwana Binte Anwar, Sadakazu Ejiri, Tadaharu Kobayashi, Hayato Ohshima, Mir Nowazesh Ali |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Bone Regeneration Radiodensity Fibrillar Collagens Osteogenesis Distraction Osteoclasts Mandible Immunoenzyme Techniques Chondrocytes Distraction Medicine Animals Rats Wistar Bone regeneration General Dentistry Endochondral ossification Fracture Healing Osteoblasts business.industry Cartilage Histology Cell Differentiation Anatomy X-Ray Microtomography Rats medicine.anatomical_structure Otorhinolaryngology Intramembranous ossification Surgery Oral Surgery business Chondrogenesis |
| Zdroj: | Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. 107(3) |
| ISSN: | 1528-395X |
| Popis: | Objective The cellular events, underlying bone regeneration through rat mandibular distraction osteogenesis (DO) was examined using micro computerized tomography (μCT), histology, and histochemistry. Study design After 5-day latency, mandibles were distracted at 0.2 mm/12 h for 10 days, and fixed at latency 5 days (L5D), distraction 3, 6, 10 days (D3D, D6D, D10D), and consolidation 1, 3, 6, 10 weeks (C1W, C3W, C6W, C10W). Results The μCT demonstrated radiopacity at the distraction gap (DG) during C1W, which was filled with new bone at C6W and C10W. At D3D, collagen fibers were aligned along the axis of the distraction vector. At D6D, alkaline phosphatase–positive osteoblasts and intramembranous ossification was observed. Collagen bundles became thicker with new bony trabeculae at D10D. Type II collagen–immunopositive areas first appeared at C1W. At C3W, cartilage tissue and endochondral ossification were found. By C6W, the entire DG had been bridged by new bone. The C10W specimens showed mature lamellar bone. Conclusion Mandibular DO produces bone through both intramembranous and endochondral ossification. |
| Databáze: | OpenAIRE |
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