Immunoprotection of Recombinant Leptospiral Immunoglobulin-Like Protein A against Leptospira interrogans Serovar Pomona Infection
| Autor: | Yung-Fu Chang, Sean P. McDonough, Ming-Jeng Pan, Mitsuharu Matsumoto, Thomas J. Divers, Chia-Sui Chen, Raghavan U.M. Palaniappan |
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| Rok vydání: | 2006 |
| Předmět: |
Serotype
Immunology Immunoglobulins Hamster Kidney Microbiology Adjuvants Immunologic Cricetinae Leptospiraceae medicine Animals Leptospirosis Staphylococcal Protein A Antigens Bacterial Vaccines Synthetic biology Antibody titer biology.organism_classification medicine.disease Virology Recombinant Proteins Disease Models Animal Leptospira interrogans serovar pomona Infectious Diseases Microbial Immunity and Vaccines biology.protein Parasitology Antibody Leptospira interrogans |
| Zdroj: | Infection and Immunity. 74:1745-1750 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.74.3.1745-1750.2006 |
| Popis: | We previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins LigA and LigB of Leptospira interrogans . LigA and LigB are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. Here, we evaluate the potential of recombinant LigA (rLigA) as a vaccine candidate against infection by L. interrogans serovar Pomona in a hamster model. rLigA was truncated into conserved (rLigAcon) and variable (rLigAvar) regions and expressed in Escherichia coli as a fusion protein with glutathione- S -transferase (rLigA). Golden Syrian hamsters were immunized at 3 and 6 weeks of age with rLigA (rLigAcon and rLigAvar) with aluminum hydroxide as an adjuvant. Hamsters given recombinant glutathione- S -transferase (rGST)-adjuvant and phosphate-buffered saline-adjuvant served as nonvaccinated controls. Three weeks after the last vaccination, all animals were challenged intraperitoneally with 10 8 L. interrogans serovar Pomona bacteria (NVSL 1427-35-093002). All hamsters immunized with recombinant LigA survived after challenge and had no significant histopathological changes. In contrast, nonimmunized and rGST-immunized hamsters were subjected to lethal doses, and the hamsters that survived showed severe tubulointerstitial nephritis. All vaccinated animals showed a rise in antibody titers against rLigA. Results from this study indicate that rLigA is a potential vaccine candidate against L. interrogans serovar Pomona infection. |
| Databáze: | OpenAIRE |
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