Reliable enzyme-linked immunosorbent assay for the determination of soybean proteins in processed foods
| Autor: | Naoki Morishita, Hiroshi Akiyama, Tadashi Ogawa, Reiko Teshima, Kumiko Kamiya, Atsuo Urisu, Tatsuya Moriyama, Shinobu Sakai, Takashi Matsumoto, Fumiki Morimatsu |
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| Rok vydání: | 2008 |
| Předmět: |
Allergy
Food Handling Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity Allergen Food allergy Food Labeling medicine Food allergens chemistry.chemical_classification Chromatography Chemistry business.industry digestive oral and skin physiology Albumin General Chemistry Allergens Antigens Plant medicine.disease Enzyme Food processing Soybean Proteins General Agricultural and Biological Sciences business Food Analysis Food Hypersensitivity |
| Zdroj: | Journal of agricultural and food chemistry. 56(16) |
| ISSN: | 1520-5118 |
| Popis: | Among allergenic foods, soybean is known as a food causing adverse reactions in allergenic patients. To clarify the validity of labeling, the specific and sensitive detection method for the analysis of the soybean protein would be necessary. The p34 protein, originally characterized to be p34 as an oil-body associated protein in soybean, has been identified as one of the major allergenic proteins and named Gly m Bd 30K. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of the soybean protein in processed foods was developed using polyclonal antibodies raised against p34 as a soybean marker protein and the specific extraction buffer for extract. The developed sandwich ELISA method was highly specific for the soybean protein. The limit of detection (LOD) and the limit of quantification (LOQ) of the developed ELISA were 0.47 ng/mL (equivalent to 0.19 microg/g in foods) and 0.94 ng/mL (equivalent to 0.38 microg/g in foods), respectively. The recovery ranged from 87.7 to 98.7%, whereas the intra- and interassay coefficients of variation were less than 4.2 and 7.5%, respectively. This study showed that the developed ELISA method is a specific, precise, and reliable tool for the quantitative analysis of the soybean protein in processed foods. |
| Databáze: | OpenAIRE |
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