P2Y receptor-mediated excitation in the posterior hypothalamus
| Autor: | Helmut L. Haas, Olga A. Sergeeva, Boris P. Klyuch, Krister S. Eriksson, Mario Siebler, Wiebke Fleischer, Tatiana Korotkova |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty P2Y receptor Thiorphan Hypothalamus Posterior Action Potentials Gene Expression Biology In Vitro Techniques Receptors Metabotropic Glutamate Internal medicine medicine Purinergic P2 Receptor Antagonists Premovement neuronal activity Animals Drug Interactions RNA Messenger Receptor Membrane potential Dose-Response Relationship Drug Adenine Nucleotides Receptors Purinergic P2 Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Methylhistamines Imidazoles Temperature Adenosine Immunohistochemistry Cell biology Rats Adenosine Diphosphate Endocrinology nervous system Animals Newborn Metabotropic glutamate receptor Pyridoxal Phosphate Gabazine GABAergic Microtubule-Associated Proteins medicine.drug Histamine |
| Zdroj: | The European journal of neuroscience. 24(5) |
| ISSN: | 0953-816X |
| Popis: | Histaminergic neurons located in the posterior hypothalamus (tuberomamillary nucleus, TMN) project widely through the whole brain controlling arousal and attention. They are tonically active during wakefulness but cease firing during sleep. As a homeostatic theory of sleep involves ATP depletion and adenosine accumulation in the brain, we investigated the role of ATP and its analogues as well as adenosine on neuronal activity in the TMN. We show increased firing of rat TMN neurons by ATP, ADP, UTP and 2meSATP, indicating activation of receptors belonging to the P2Y family. Adenosine affected neither membrane potential nor firing of these cells. Single-cell reverse transcriptase-polymerase chain reaction revealed that P2Y1 and P2Y4 are prevailing receptors in TMN neurons. P2Y1 receptor mRNA was detected with a higher frequency in 2-week-old than in 4-week-old rats; in accordance, 2meSATP was more potent than ATP. Semi-quantitative real-time polymerase chain reaction revealed a developmental downregulation of mRNA levels for P2Y1 and P2Y4 receptors. Immunocytochemistry demonstrated neuronal and glial localization of the P2Y1 receptor protein. Network activity measured with multielectrode arrays in primary cultures made from the posterior hypothalamus was enhanced by UTP and 2meSATP (P2Y4 and P2Y1 agonists, respectively). ATP caused an inhibition of firing, which was reversed in the presence of suramin or gabazine [gamma-aminobutyric acid (GABA)A receptor antagonist], indicating that GABAergic neurons are preferentially activated by ATP in this network. Excitation of the wake-active TMN neurons by nucleotides and the lack of adenosine action may be important factors in sleep-wake regulation. |
| Databáze: | OpenAIRE |
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