An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle
| Autor: | Beatriz S. Valentini, Marcelo Fort, Lucía María Campero, Luis Miguel Ortega-Mora, Gema Álvarez-García, Marcos Enrique Serrano-Martínez, Dora Cano, Rinaldo Aparecido Mota, Magdalena Rambeaud, Carlos Cruz-Vázquez, María Cecilia Venturini, Dadin Prando Moore, Ignacio Echaide, Carlos Manuel Campero, Andrea Dellarupe, Javier Moreno-Gonzalo, Gastón Moré |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Veterinary medicine Argentina Ibero-America Antibodies Protozoan Cattle Diseases Enzyme-Linked Immunosorbent Assay Target population Serological assay trial Serology 03 medical and health sciences Food Animals Otras Ciencias Veterinarias Peru parasitic diseases Animals Medicine Serologic Tests Inter-laboratory Fluorescent Antibody Technique Indirect Mexico biology Coccidiosis business.industry Ciencias Veterinarias Neospora 030108 mycology & parasitology biology.organism_classification Neospora caninum Spain CIENCIAS AGRÍCOLAS biology.protein purl.org/pe-repo/ocde/ford#4.03.00 [https] Cattle Animal Science and Zoology Bovine neosporosis Antibody business Area under the roc curve Brazil |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero- American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the BPre-test information,^ which uses previous epidemiological and serological data; (2) the BMajority of tests,^ which classifies a serumas positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. Facultad de Ciencias Veterinarias |
| Databáze: | OpenAIRE |
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