Use of multigeneration-family molecular dog leukocyte antigen typing to select a hematopoietic cell transplant donor for a dog with T-cell lymphoma
| Autor: | Edmund W. Sullivan, Peter F Moore, Eustacia Zellmer, Rainer Storb, Theresa E. Westfall, B. J. Weigler, Marie Térèse Little, Marilena Lupu, Christian S. Kuhr, Patrice A. Stroup |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Lymphoma T-Cell Dogs Immunophenotyping Histocompatibility Antigens Granulocyte Colony-Stimulating Factor medicine Animals Transplantation Homologous T-cell lymphoma Dog Diseases Immunosuppression Therapy Transplantation Chimera General Veterinary biology business.industry Histocompatibility Testing Dog leukocyte antigen Graft Survival Hematopoietic Stem Cell Transplantation Leukapheresis Total body irradiation medicine.disease Pancytopenia Hematopoietic Stem Cell Mobilization Lymphoma Treatment Outcome medicine.anatomical_structure Cyclosporine biology.protein Bone marrow business Whole-Body Irradiation |
| Zdroj: | Journal of the American Veterinary Medical Association. 228:728-732 |
| ISSN: | 0003-1488 |
| DOI: | 10.2460/javma.228.5.728 |
| Popis: | Case Description—A 7-year-old Golden Retriever was examined because of anorexia, lethargy, vomiting, and gradual weight loss. Clinical Findings—Splenomegaly, pancytopenia, high serum calcium concentration, and high alkaline phosphatase activity were detected. Magnetic resonance imaging revealed an enlarged mesenteric lymph node and increased signals from the bone marrow of the ilium and vertebral bodies. Histologic examination and immunophenotyping of biopsy specimens confirmed a stage V (b) T-cell malignant lymphoma. Treatment and Outcome—Clinical remission was attained by use of 2 chemotherapy cycles, followed by an allogeneic hematopoietic cell transplant performed at 18 weeks after diagnosis. A donor was identified by molecular dog leukocyte antigen typing methods. The patient was conditioned with 2 fractions of 4 Gy total body irradiation delivered 3 hours apart at 7 cGy/min, followed by an IV infusion of recombinant canine granulocyte colony-stimulating factor mobilized leukapheresis product and postgrafting immunosuppression with cyclosporine. Chimerism analyses revealed full donor engraftment that has been maintained for at least 58 weeks after transplant. Remission has been confirmed by normal results of serum thymidine kinase assays and the absence of peripheral blood clonal T-cell receptor gene rearrangements. Clinical Relevance—Systemic chemotherapy induces remissions; however, most dogs succumb to disease recurrence because of multidrug resistance. Outcome of allogeneic hematopoietic cell transplantation in dogs can be excellent because of improved donor-recipient selection by use of molecular dog leukocyte antigen typing, compared with early attempts, and better prevention of graft versus host disease, better supportive care, and substitution of peripheral blood mononuclear cells for bone marrow. |
| Databáze: | OpenAIRE |
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