Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos
Autor: | Onanong Sengvilaipaseuth, Audrey Dubot-Pérès, Ooyanong Phonemixay, Paul N. Newton, Anisone Chanthongthip, Yixiao Lu, Manivanh Vongsouvath, Stuart D. Blacksell, Phonelavanh Phouminh |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
medicine.medical_specialty IgM viruses 030106 microbiology Mac elisa lcsh:Medicine serology Context (language use) Dengue virus medicine.disease_cause Article Serology Dengue fever 03 medical and health sciences Internal medicine medicine General Immunology and Microbiology Capture elisa business.industry lcsh:R Public Health Environmental and Occupational Health medicine.disease dengue 3. Good health 030104 developmental biology Infectious Diseases Igm elisa Laos Who criteria MAC-ELISA business |
Zdroj: | Tropical Medicine and Infectious Disease Volume 4 Issue 3 Tropical Medicine and Infectious Disease, Vol 4, Iss 3, p 111 (2019) |
ISSN: | 2414-6366 |
DOI: | 10.3390/tropicalmed4030111 |
Popis: | The endemicity of Dengue virus (DENV) infection remains a major public health problem in Lao PDR. In this study, we compared two commercial anti-dengue IgM ELISA kits, Panbio® Dengue IgM Capture ELISA (Panbio Kit, Alere, Waltham, MA, USA) and DEN DetectTM MAC-ELISA (InBios kit, InBios International, Inc., Seattle, WA, USA), in the context of diagnosis of patients admitted to hospital with clinical dengue presentation. Two panels of paired blood samples were tested. Panel A was composed of 54 dengue confirmed patients (by DENV real-time RT-PCR) and 11 non-dengue dengue patients (other infections confirmed by corresponding PCR results). Panel B included 74 patients randomly selected from consecutive patients admitted to Mahosot Hospital in 2008 with suspicion of dengue fever according to WHO criteria. Results from panel A showed significantly better sensitivity for Panbio kit (64.8% 95%CI: 50.6&ndash 77.3%) than for InBios kit (18.5% 95%CI: 9.3&ndash 31.4%) when testing admission sera. Sensitivity was increased for both kits when combining results from admission and convalescent sera. Concordant results were obtained from panel B with fair agreement (&kappa = 0.29) between both kits when testing single admission samples, and moderate agreement (&kappa = 0.5) when combining results from admission and convalescent sera. |
Databáze: | OpenAIRE |
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