Analysis of miRNA-mRNA Crosstalk in Radiation-Induced Mouse Thymic Lymphomas to Identify miR-486 as a Critical Regulator by Targeting IGF2BP3 mRNA
| Autor: | Hainan Zhao, Suhe Dong, Jicong Du, Penglin Xia, Ruling Liu, Tingting Liu, Yajie Yang, Ying Cheng, Jianming Cai, Cong Liu, Fu Gao, Hu Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Regulator Biology medicine.disease_cause lcsh:RC254-282 Metastasis 03 medical and health sciences 0302 clinical medicine microRNA medicine radiation-induced thymic lymphoma Thymic Lymphoma Original Research IGF2BP3 medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens miR-486 Lymphoma Crosstalk (biology) 030104 developmental biology Oncology miRNA-mRNA regulatory network 030220 oncology & carcinogenesis Knockout mouse Cancer research Carcinogenesis ionizing radiation |
| Zdroj: | Frontiers in Oncology Frontiers in Oncology, Vol 10 (2021) |
| ISSN: | 2234-943X |
| Popis: | Ionizing radiation is one of the common environmental carcinogens. miRNAs play critical roles in the processes of tumor occurrence, development, metastasis. However, the relationship between radiation-induced carcinogenesis and miRNA rarely reported. This study is aimed to investigate the effect of miRNAs on radiation-induced carcinogenesis. In this study we established the radiation-induced thymic lymphoma mice model. By using miRNA array of RTL tissue and predicting for miRNAs target genes, a miRNA-mRNA crosstalk network was established. Based on this network, we identified a critical miRNA, miR-486, which was the most down-regulated in the radiation-induced carcinogenesis. Then the function of miR-486 was confirmed by using knockout mice and cellular experiments. As a result, miR-486 could inhibit proliferation of mouse lymphoma cells by targeting IGF2BP3 mRNA. The adenovirus over-expression miR-486 vector reduced tumorigenesis in vivo. MiR-486 knockout mice have a strong tendency of radiation-induced carcinogenesis. In conclusion, miR-486 inhibits the proliferation of lymphoma cells and tumorigenesis induced by radiation through targeting IGF2BP3. |
| Databáze: | OpenAIRE |
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