Quantitative GC determination of sodium triacetoxyborohydride (STAB)
Autor: | Robert Wethman, Ian Hale, James Chadwick |
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Rok vydání: | 2021 |
Předmět: |
Degradation kinetics
Reducing agent Clinical Biochemistry Pharmaceutical Science 01 natural sciences Reductive amination Analytical Chemistry chemistry.chemical_compound Drug Discovery Derivatization Hplc method Spectroscopy Chromatography High Pressure Liquid Amination Chromatography 010405 organic chemistry 010401 analytical chemistry Sodium 0104 chemical sciences Stab body regions Sodium triacetoxyborohydride chemistry Reagent cardiovascular system Indicators and Reagents |
Zdroj: | Journal of pharmaceutical and biomedical analysis. 203 |
ISSN: | 1873-264X |
Popis: | Sodium triacetoxyborohydride (STAB) is a mild, selective, and frequently used reducing agent for reductive amination transformations. STAB has been reported to undergo degradation when exposed to air, often requiring additional sub-stoichiometric reagent charges to drive reactions to completion. We report the development and qualification of a rapid, quantitative GC derivatization method using 3,4-dihydroisoquinoline to enable accurate hydride assay determination of STAB. Assay data generated from this method has been directly compared to a recently published HPLC method using bromosalicaldehyde as the derivatization reagent. The degradation kinetics of STAB have also been further investigated by Raman spectroscopy to demonstrate how exposure of STAB to air can negatively impact the reagent’s performance. |
Databáze: | OpenAIRE |
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