Snake Venom Gland Organoids
| Autor: | Post, Yorick, Puschhof, Jens, Beumer, Joep, Kerkkamp, Harald M, de Bakker, Merijn A G, Slagboom, Julien, de Barbanson, Buys, Wevers, Nienke R, Spijkers, Xandor M, Olivier, Thomas, Kazandjian, Taline D, Ainsworth, Stuart, Iglesias, Carmen Lopez, van de Wetering, Willine J, Heinz, Maria C, van Ineveld, Ravian L, van Kleef, Regina G D M, Begthel, Harry, Korving, Jeroen, Bar-Ephraim, Yotam E, Getreuer, Walter, Rios, Anne C, Westerink, Remco H S, Snippert, Hugo J G, van Oudenaarden, Alexander, Peters, Peter J, Vonk, Freek J, Kool, Jeroen, Richardson, Michael K, Casewell, Nicholas R, Clevers, Hans, Landdegradatie en aardobservatie, One Health Toxicologie, dIRAS RA-1, Sub Developmental Biology, Dep Farmaceutische wetenschappen, Developmental Biology |
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| Přispěvatelé: | Landdegradatie en aardobservatie, One Health Toxicologie, dIRAS RA-1, Sub Developmental Biology, Dep Farmaceutische wetenschappen, Developmental Biology, Faculteit FHML Centraal, Institute of Nanoscopy (IoN), RS: M4I - Nanoscopy, BioAnalytical Chemistry, AIMMS, Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell Culture Techniques
Venom Stem cell marker Salivary Glands Transcriptome transcriptomics 0302 clinical medicine 0303 health sciences snake Stem Cells TERM PRIMARY CULTURE LGR5 Snakes knock-in reporter Cell biology Organoids RECEPTORS Adult Stem Cells Snake venom Stem cell STEM-CELLS Adult stem cell wd_410 Snake Venoms PROTEINS organoid qu_475 Coral Snakes Biology SECRETORY-CELLS complex mixtures General Biochemistry Genetics and Molecular Biology Lgr5 03 medical and health sciences stem cells single cell RNA sequencing Organoid Animals SDG 14 - Life Below Water 030304 developmental biology Toxins Biological venom gland IDENTIFICATION Gene Expression Profiling qu_300 IN-VITRO NEUROTOXINS EVOLUTION qu_550 heterogeneity 030217 neurology & neurosurgery |
| Zdroj: | Cell, 180(2), 233-247.e21. Cell Press Post, Y, Puschhof, J, Beumer, J, Kerkkamp, H M, de Bakker, M A G, Slagboom, J, de Barbanson, B, Wevers, N R, Spijkers, X M, Olivier, T, Kazandjian, T D, Ainsworth, S, Iglesias, C L, van de Wetering, W J, Heinz, M C, van Ineveld, R L, van Kleef, R G D M, Begthel, H, Korving, J, Bar-Ephraim, Y E, Getreuer, W, Rios, A C, Westerink, R H S, Snippert, H J G, van Oudenaarden, A, Peters, P J, Vonk, F J, Kool, J, Richardson, M K, Casewell, N R & Clevers, H 2020, ' Snake Venom Gland Organoids ', Cell, vol. 180, no. 2, pp. 233-247.e21 . https://doi.org/10.1016/j.cell.2019.11.038 Cell, 180(2), 233. Cell Press Cell, 180(2), 233-247.e21. Elsevier B.V. CELL, 180(2), 233-247. CELL PRESS |
| ISSN: | 0092-8674 |
| Popis: | Wnt dependency and Lgr5 expression define multiple mammalian epithelial stem cell types. Under defined growth factor conditions, such adult stem cells (ASCs) grow as 3D organoids that recapitulate essential features of the pertinent epithelium. Here, we establish long-term expanding venom gland organoids from several snake species. The newly assembled transcriptome of the Cape coral snake reveals that organoids express high levels of toxin transcripts. Single-cell RNA sequencing of both organoids and primary tissue identifies distinct venom-expressing cell types as well as proliferative cells expressing homologs of known mammalian stem cell markers. A hard-wired regional heterogeneity in the expression of individual venom components is maintained in organoid cultures. Harvested venom peptides reflect crude venom composition and display biological activity. This study extends organoid technology to reptilian tissues and describes an experimentally tractable model system representing the snake venom gland. |
| Databáze: | OpenAIRE |
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